LOCUS       sequence-121055-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His-MBP-Smt3-NpuDnaEN102-CBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Guerrero F, Ciragan A, Iwai H
  TITLE     Tandem SUMO fusion vectors for improving soluble protein expression 
            and purification.
  JOURNAL   Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019.
  PUBMED    26297996
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019."
COMMENT     Sequence Label: pLJSRSF7
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..52)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(81..236)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
ORIGIN
        1 tagtagacga gtccatgtgc tggcgttcaa atttcgcagc agcggtttct ttaccagact
       61 cgagtgcggc cgcaagctta ttgaagctgc cacaaggcag gaacgttgga tggttcccat
      121 cctgccaagg aggtgtgggg ctgcaaacat ttatacgtct tgccgttata tgtgaccaat
      181 tgtcccgcag tataagctgt gttgacctgc caagcggata caccaggatt tgtggtaccg
      241 aattcgttcg gcaaattatc aacccgcatc aaatccaatt cacgttcaaa tatttcatca
      301 attggcaaca tttgaccatc aacagtcata aacttatggt cttttgttgc ccgaatcaat
      361 gaaccatctt ccaaacaata ctcaaacacc tcttgttctc cgcgatcgtg ccattgtgct
      421 acaggttgtg tataaatatt tccattatta tcaacgctat aaacagtaca ttcgatgcgc
      481 ttttctacaa ttttaccaat cggtaataat ccatattcta ctgtcaatat ttccgtttca
      541 tagcttaaac aggatccttc cgttacggtg taggttttgg tagcgtcgtc gtaggtccat
      601 tcaccgtcaa caccgttgtc gttagcgtac tgtttgaaaa ctttttccgc ggtagcagcg
      661 tcaacagctt cggtggtggt ttcacctttc agggttttac cgttcaggat aagtttgtac
      721 tgcatatggg atccaccaat ctgttctctg tgagtctcaa taatatcgtt atcctccatg
      781 tccaaatctt caggggtctg atcagcttga attctaatac cgtcgtacaa gaatcttaag
      841 gagtccattt ccttaccctg tcttttagcg aacgc
//
