LOCUS       sequence-121060-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Middle entry nuclear localized tdTomato reporter with 6xMT and 2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND
  TITLE     Vegfa signals through ERK to promote angiogenesis, but not artery 
            differentiation.
  JOURNAL   Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30.
  PUBMED    27578780
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3796-3805. Epub 2016 Aug 30"
COMMENT     Sequence Label: pME-6MTnlstdTomato2A
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..119
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(121..177)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(471..490)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     740..758
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggggatatca gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt
       61 gcaacaaatt gataagcaat gctttcttat aatgccaact ttgtacaaga aagctgggtg
      121 aggaccgggg ttttcttcca cgtctcctgc ttgctttaac agagagaagt tcgtggctcc
      181 ggatcctccc ttgtacagct cgtccatgcc gtacaggaac aggtggtggc ggccctcgga
      241 gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggt
      301 gtccacgtag tagtagccgg gcagttgcac gggcttcttg gccatgtaga tggtcttgaa
      361 ctccaccagg tagtggccgc cgtccttcag cttcagggcc tggtggatct cgcccttcag
      421 cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg
      481 cattacgggg ccgtcggggg ggaagttggt gccgcgcatc ttcaccttgt agatcagcgt
      541 gccgtcctgc agggaggagt cctgggtcac ggtcaccaga ccgccgtcct cgaagttcat
      601 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtaatcgg ggatgtcggc
      661 ggggtgcttc acgtacgcct tggagccgta catgaactgg ggggacagga tgtcccaggc
      721 gaagggcagg gggccgccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
      781 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcatggagc cctccatgcg
      841 caccttgaag cgcatgaact ctttgatgac ggccatgttg ttgtcctcgg aggaggcggt
      901 gccggagctg ccgctgccgg tgctgccggt gccatgcccc aggaacaggt ggtggcggcc
      961 ctcggagcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
     1021 cttggtgtcc a
//
