LOCUS       sequence-121064-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal 
            expression in C. Elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Nguyen JP, Shipley FB, Linder AN, Plummer GS, Liu M, Setru SU, 
            Shaevitz JW, Leifer AM
  TITLE     Whole-brain calcium imaging with cellular resolution in freely 
            behaving Caenorhabditis elegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Dec 28. pii: 201507110.
  PUBMED    26712014
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Dec 28. pii: 201507110."
COMMENT     Sequence Label: Prab-3::NLS::GCaMP6s
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(93..114)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(327..383)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(411..425,426..434))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(438..470)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(474..491)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(501..521)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc
       61 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      121 cgcttctcgt tggggtcttt cgaaagtttg gactgcacgc tcaggtagtg gttgtcgggc
      181 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg
      241 ccgccgtcct cgatgttgtg gcggatgtgg aagttcgcct tgatgccgtt cttctgcttg
      301 tcggccttga tatagacgtt ctcgagtgag ctcagccgac ctatagctct gactgcgtga
      361 cctgtcttat tccacttacg acgtgatgag tcgaccatgg tggcgagatc cttatcgtca
      421 tcgtcgtaca gatcccgacc catttgctgt ccaccagtca tgctagccat accatgatga
      481 tgatgatgat gagaacccat taccttacgc ttcttctttg gagccatgga tccctgaaaa
      541 tagggctact gtagatttat tttaaaagag catttagatt actttatgta tttatagaat
      601 caaaatatca aaaattcaaa gttcatagcg agatatcttt gattaaaaat tagagaagga
      661 agaaaaactt gcaacaaaaa aggaaataag aaatgtctgg agaattcaag attagacaga
      721 aaaaaacgtt atttccattt tccaatttta atttcccaac ccaacaacaa aacttagaga
      781 aacgagaggg aactgattaa aatagcaaat ttttatcatt ctttttattt ctctagtgta
      841 tttatcagct gtctctctaa aactctttct ctggaggaaa aattgaagtt agtggactac
      901 aactcgttgg gaaaa
//
