LOCUS       sequence-121072-        4935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ZF9 Op x6 -- NTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pGL4.26-SS-358
FEATURES             Location/Qualifiers
     source          1..4935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        126..157
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    890..1011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(901..920)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     955..974
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1205..1562
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1205..1396
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1228..1248)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1413..1548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1475..1494
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1593..2630
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             2609..2617
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    2654..2702
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(2858..2875)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3029..3617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3109..3128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3817..4608,4609..4677))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4782..4830
                     /note="synthetic polyadenylation signal"
     misc_feature    4844..4935
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4884..4903
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gctgtagatg gagaaatgta gatggagaaa
       61 tgtagatgga gaaatgtaga tggagaaatg tagatggaga aatgtagatg gagaagctta
      121 gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg tactgttggt
      181 aaagccaccc atatggacat catcagcgtg gctctgaaga ggcactccac caaggctttc
      241 gacgcttcca agaaactgac ccctgaacag gccgagcaga tcaagaccct gctccagtac
      301 agccctagct ccaccaacag ccagccttgg cacttcatcg tggctagcac cgaggaaggc
      361 aaagctaggg tggctaagag cgccgctggc aactacgtgt tcaacgagag gaagatgctg
      421 gatgctagcc acgtggtggt gttctgcgct aagaccgcca tggacgatgt gtggctgaag
      481 ctggtggtgg atcaggaaga tgctgatggc aggttcgcta cccctgaagc taaggccgct
      541 aacgacaagg gcaggaagtt cttcgccgac atgcacagga aggatctgca cgatgatgct
      601 gagtggatgg ccaagcaggt gtacctgaac gtgggcaact tcctgctcgg cgtggctgcc
      661 ctgggcctcg atgctgtgcc catcgaaggc ttcgatgctg ctatcctgga tgccgagttc
      721 ggcctgaagg agaaaggcta caccagcctg gtggtggtgc ctgtgggcca ccacagcgtg
      781 gaggacttca acgctaccct gcctaagagc aggctgcccc agaacatcac cctgaccgag
      841 gtgtgataat tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt
      901 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
      961 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1021 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     1081 taaaacctct acaaatgtgg taaaatcgat aaggatccgt ttgcgtattg ggcgctcttc
     1141 cgctgatctg cgcagcacca tggcctgaaa taacctctga aagaggaact tggttagcta
     1201 ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     1261 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     1321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1381 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1441 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1501 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     1561 aaaagctcga ttcttctgac actagcgcca ccatgaagaa gcccgaactc accgctacca
     1621 gcgttgaaaa atttctcatc gagaagttcg acagtgtgag cgacctgatg cagttgtcgg
     1681 agggcgaaga gagccgagcc ttcagcttcg atgtcggcgg acgcggctat gtactgcggg
     1741 tgaatagctg cgctgatggc ttctacaaag accgctacgt gtaccgccac ttcgccagcg
     1801 ctgcactacc catccccgaa gtgttggaca tcggcgagtt cagcgagagc ctgacatact
     1861 gcatcagtag acgcgcccaa ggcgttactc tccaagacct ccccgaaaca gagctgcctg
     1921 ctgtgttaca gcctgtcgcc gaagctatgg atgctattgc cgccgccgac ctcagtcaaa
     1981 ccagcggctt cggcccattc gggccccaag gcatcggcca gtacacaacc tggcgggatt
     2041 tcatttgcgc cattgctgat ccccatgtct accactggca gaccgtgatg gacgacaccg
     2101 tgtccgccag cgtagctcaa gccctggacg aactgatgct gtgggccgaa gactgtcccg
     2161 aggtgcgcca cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc gacaacggcc
     2221 gcatcaccgc cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag tacgaggtgg
     2281 ccaacatctt cttctggcgg ccctggctgg cttgcatgga gcagcagact cgctacttcg
     2341 agcgccggca tcccgagctg gccggcagcc ctcgtctgcg agcctacatg ctgcgcatcg
     2401 gcctggatca gctctaccag agcctcgtgg acggcaactt cgacgatgct gcctgggctc
     2461 aaggccgctg cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc acacaaatcg
     2521 ctcgccggag cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc gacagcggca
     2581 accgccggcc cagtacacga ccgcgcgcta aggaggtagg tcgagtttaa actctagaac
     2641 cggtcatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     2701 tgttcgaact agatgctgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     2761 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     2821 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3781 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     3841 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     3901 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     3961 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     4021 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     4081 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     4141 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     4201 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     4261 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     4321 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     4381 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     4441 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     4501 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     4561 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     4621 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     4681 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     4741 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     4801 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     4861 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     4921 ccagaacatt tctct
//
