LOCUS       sequence-121074-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H6-TRX-Smt3-GB1-NpuDnaEN102-CBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Guerrero F, Ciragan A, Iwai H
  TITLE     Tandem SUMO fusion vectors for improving soluble protein expression 
            and purification.
  JOURNAL   Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019.
  PUBMED    26297996
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019."
COMMENT     Sequence Label: pCARSF63
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..387
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     CDS             394..684
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
ORIGIN
        1 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 ggcagcgata aaattattca cctgactgac gacagttttg acacggatgt actcaaagcg
      121 gacggggcga tcctcgtcga tttctgggca gagtggtgcg gtccgtgcaa aatgatcgcc
      181 ccgattctgg atgaaatcgc tgacgaatat cagggcaaac tgaccgttgc aaaactgaac
      241 atcgatcaaa accctggcac tgcgccgaaa tatggcatcc gtggtatccc gactctgctg
      301 ctgttcaaaa acggtgaagt ggcggcaacc aaagtgggtg cactgtctaa aggtcagttg
      361 aaagagttcc tcgacgctaa cctggcggga actagtgact cagaagtcaa tcaagaagct
      421 aagccagagg tcaagccaga agtcaagcct gagactcaca tcaatttaaa ggtgtccgat
      481 ggatcttcag aaatcttctt taagatcaaa aagaccactc ctttaagaag gctgatggaa
      541 gcgttcgcta aaagacaggg taaggaaatg gactccttaa gattcttgta cgacggtatt
      601 agaattcaag ctgatcagac ccctgaagat ttggacatgg aggataacga tattattgag
      661 actcacagag aacagattgg tggatcccat atgcagtaca aacttatcct gaacggtaaa
      721 accctgaaag gtgaaaccac caccgaagct gttgacgctg ctaccgcgga aaaagttttc
      781 aaacag
//
