LOCUS       sequence-121076-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H6-TRX-Smt3-GB1-NpuDnaEN102-CBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Guerrero F, Ciragan A, Iwai H
  TITLE     Tandem SUMO fusion vectors for improving soluble protein expression 
            and purification.
  JOURNAL   Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019.
  PUBMED    26297996
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019."
COMMENT     Sequence Label: pCARSF63
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(78..233)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gtgcggccgc aagcttattg aagctgccac aaggcaggaa cgttggatgg ttcccatcct
      121 gccaaggagg tgtggggctg caaacattta tacgtcttgc cgttatatgt gaccaattgt
      181 cccgcagtat aagctgtgtt gacctgccaa gcggatacac caggatttgt ggtaccgaat
      241 tcgttcggca aattatcaac ccgcatcaaa tccaattcac gttcaaatat ttcatcaatt
      301 ggcaacattt gaccatcaac agtcataaac ttatggtctt ttgttgcccg aatcaatgaa
      361 ccatcttcca aacaatactc aaacacctct tgttctccgc gatcgtgcca ttgtgctaca
      421 ggttgtgtat aaatatttcc attattatca acgctataaa cagtacattc gatgcgcttt
      481 tctacaattt taccaatcgg taataatcca tattctactg tcaatatttc cgtttcatag
      541 cttaaacagg atccttccgt tacggtgtag gttttggtag cgtcgtcgta ggtccattca
      601 ccgtcaacac cgttgtcgtt agcgtactgt ttgaaaactt tttccgcggt agcagcgtca
      661 acagcttcgg tggtggtttc acctttcagg gttttaccgt tcaggataag tttgtactgc
      721 atatgggatc caccaatctg ttctctgtga gtctcaataa tatcgttatc ctccatgtcc
      781 aaatcttcag gggtctgatc agcttgaatt ctaataccgt cgtacaagaa tc
//
