LOCUS       sequence-121084-       11368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-338
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     CDS             1585..1608
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1609..1629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2343..2354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2467..2616
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    2635..3079
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     CDS             3110..3820
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWQASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3255..3273)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3523..3542
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3848..3969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4157..4745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4646..4665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4820..4868
                     /note="synthetic polyadenylation signal"
     misc_feature    4882..4973
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4922..4941
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        5099..5130
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      5157..5166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5163..5165,5166..5168,5169..5882)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5163..5165,5166..5168,5169..5879)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5208..5229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5469..5488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5816..5837
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5926..6047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6159..6462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6577..7882
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    6919..7850
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             7898..7921
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7922..7942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    9411..9855
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        9888..9934
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        9926..9944
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9954..10748
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9954..10748
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10008..10027)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10618..10637
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10795..11367
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaggatc catggactac aaagacgatg acgacaagcc taagaagaaa
     1621 cggaaggtgt ccagacctgg ggagaggcca ttccagtgtc ggatctgtat gagaaatttt
     1681 agcactatgg ctgtactgag gagacataca agaacccata caggcgagaa gccctttcag
     1741 tgcaggatct gcatgcgaaa cttttctcgc agagaagtgc ttgaaaacca cctgcggact
     1801 cataccggcg aaaaaccatt tcagtgtcga atttgcatgc gcaactttag ccagacagtg
     1861 aacttggata gacaccttaa gactcatctg aggcagaagg acggggagag acctttccag
     1921 tgcagaatct gcatgagaaa cttttctaag aaagatcatc tgcaccgcca tacccgcacc
     1981 cacaccggcg aaaaaccctt ccagtgccgc atctgtatgc gaaattttag ccagaggcca
     2041 catcttacaa atcaccttag aacacacact ggagaaaaac catttcagtg tcggatctgc
     2101 atgcgcaact tctccgtggg cgccagcctc aaacgccacc tgaaaaccca cctgagggga
     2161 tctgggggcg gatcaggtgg aggctcagga ggtgggtccg gaggtggatc tggtggcggg
     2221 tccggaggcg gatccgtttt gcttaacgtt ctttcgaagt gtgccggctc taaaaaattc
     2281 aggcctgctc ccgccgctgc ttttgcacgt gagtgccgcg gattttattt cgagttacaa
     2341 gaattgaagg aagacgatta ttatgggatt actttatctg atgattctga tcatcagttt
     2401 ttgcttgcca accaggttgt cgtccataat tgcaccatga ccgagaaggg cagcggaggg
     2461 cgcgccgacg cgctggacga tttcgatctc gacatgctgg gttctgatgc cctcgatgac
     2521 tttgacctgg atatgttggg aagcgacgca ttggatgact ttgatctgga catgctcggc
     2581 tccgatgctc tggacgattt cgatctcgat atgttaatta attgttaagc tagcagcagg
     2641 tttccccaat gacacaaaac gtgcaacttg aaactccgcc tggtctttcc aggtctagag
     2701 gggtaacact ttgtactgcg tttggctcca cgctcgatcc actggcgagt gttagtaaca
     2761 gcactgttgc ttcgtagcgg agcatgacgg ccgtgggaac tcctccttgg taacaaggac
     2821 ccacggggcc aaaagccacg cccacacggg cccgtcatgt gtgcaacccc agcacggcga
     2881 ctttactgcg aaacccactt taaagtgaca ttgaaactgg tacccacaca ctggtgacag
     2941 gctaaggatg cccttcaggt accccgaggt aacacgcgac actcgggatc tgagaagggg
     3001 actggggctt ctataaaagc gctcggttta aaaagcttct atgcctgaat aggtgaccgg
     3061 aggtcggcac ctttcctttg caattactga ccctatgaat acactcgaga tggtgagcaa
     3121 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     3181 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3241 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     3301 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     3361 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3421 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     3481 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     3541 gaccatgggc tggcaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     3601 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     3661 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     3721 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     3781 ccgccactcc accggcggca tggacgagct gtacaagtaa gcggccgcac tagctggcca
     3841 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3901 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3961 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4021 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggaaatgtt aattaactag
     4081 ccatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4801 acatgttctt gtttaaacta ataaaatatc tttattttca ttacatctgt gtgttggttt
     4861 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     4921 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa
     4981 ctggccggta cctgagctcg ctagctgtag atggagaaat gtagatggag aaatgtagat
     5041 ggagaaatgt agatggagaa atgtagatgg agaaatgtag atggagaagc ttagacacta
     5101 gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca
     5161 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5221 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5281 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5341 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5401 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5461 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5521 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5581 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5641 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5701 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5761 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5821 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5881 aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     5941 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     6001 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     6061 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     6121 acctctacaa atgtggtatc atgaaattaa cctgcaggcg ttacataact tacggtaaat
     6181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     6241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     6301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     6361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     6421 acttggcagt acatctacgt attagtcatc gctattacca tgatgatgcg gttttggcag
     6481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     6541 gacgtcaatg ggagtttgtt ttgactagtg gagccgagag taattcatac aaaaggaggg
     6601 atcgccttcg caaggggaga gcccagggac cgtccctaaa ttctcacaga cccaaatccc
     6661 tgtagccgcc ccacgacagc gcgaggagca tgcgcccagg gctgagcgcg ggtagatcag
     6721 agcacacaag ctcacagtcc ccggcggtgg ggggaggggc gcgctgagcg ggggccaggg
     6781 agctggcgcg gggcaaactg ggaaagtggt gtcgtgtgct ggctccgccc tcttcccgag
     6841 ggtgggggag aacggtatat aagtgcggta gtcgccttgg acgttctttt tcgcaacggg
     6901 tttgccgtca gaacgcaggt gagtggcggg tgtggcttcc gcgggccccg gagctggagc
     6961 cctgctctga gcgggccggg ctgatatgcg agtgtcgtcc gcagggttta gctgtgagca
     7021 ttcccacttc gagtggcggg cggtgcgggg gtgagagtgc gaggcctagc ggcaaccccg
     7081 tagcctcgcc tcgtgtccgg cttgaggcct agcgtggtgt ccgccgccgc gtgccactcc
     7141 ggccgcacta tgcgtttttt gtccttgctg ccctcgattg ccttccagca gcatgggcta
     7201 acaaagggag ggtgtggggc tcactcttaa ggagcccatg aagcttacgt tggataggaa
     7261 tggaagggca ggaggggcga ctggggcccg cccgccttcg gagcacatgt ccgacgccac
     7321 ctggatgggg cgaggcctgt ggctttccga agcaatcggg cgtgagttta gcctacctgg
     7381 gccatgtggc cctagcactg ggcacggtct ggcctggcgg tgccgcgttc ccttgcctcc
     7441 caacaagggt gaggccgtcc cgcccggcac cagttgcttg cgcggaaaga tggccgctcc
     7501 cggggccctg ttgcaaggag ctcaaaatgg aggacgcggc agcccggtgg agcgggcggg
     7561 tgagtcaccc acacaaagga agagggcctt gcccctcgcc ggccgctgct tcctgtgacc
     7621 ccgtggtcta tcggccgcat agtcacctcg ggcttctctt gagcaccgct cgtcgcggcg
     7681 gggggagggg atctaatggc gttggagttt gttcacattt ggtgggtgga gactagtcag
     7741 gccagcctgg cgctggaagt cattcttgga atttgcccct ttgagtttgg agcgaggcta
     7801 attctcaagc ctcttagcgg ttcaaaggta ttttctaaac ccgtttccag gtgttgtgaa
     7861 agccaccgct aattcaaagc aatccggagt atacatggac tacaaggacg acgatgacaa
     7921 gcccaagaaa aagcgcaagg tctctagacc cggggagcgc cccttccagt gtcgcatttg
     7981 catgcggaac ttttcggata aaactaaatt gagagttcat acccgtactc ataccggtga
     8041 aaaaccgttt cagtgtcgga tctgtatgcg aaatttctcc gttagacata atttgactag
     8101 acatctacgt acgcacaccg gcgagaagcc attccaatgc cgaatatgca tgcgcaactt
     8161 cagtcaatct acttctttgc aaagacacct aaaaacccac ctgagaggat ccggcggagt
     8221 gctggagaag ggctgctttg ccaagggtac caatgtttta atggcggatg ggtctattga
     8281 atgtattgaa aacattgagg ttggtaataa ggtcatgggt aaagatggca gacctcgtga
     8341 ggtaattaaa ttgcccagag gaagagaaac tatgtacagc gtcgtgcaga aaagtcagca
     8401 cagagcccac aaaagtgact caagtcgtga agtgccagaa ttactcaagt ttacgtgtaa
     8461 tgcgacccat gagttggttg ttagaacacc tcgtagtgtc cgccgtttgt ctcgtaccat
     8521 taagggtgtc gaatattttg aagttattac ttttgagatg ggccaaaaga aagcccccga
     8581 cggtagaatt gttgagcttg tcaaggaagt ttcaaagagc tacccaatat ctgaggggcc
     8641 tgagagagcc aacgaattag tagaatccta tagaaaggct tcaaataaag cttattttga
     8701 gtggactatt gaggccagag atctttctct gttgggttgc cacgttcgca aagccactta
     8761 tcagacatac gccccaatcg gcgggggttc tgggggagga tcaggtggtg ggtccggggg
     8821 aggaagcggg ggtggctctg ggggtggatc aacacgtccc ggggagcgcc ccttccagtg
     8881 caggatctgc atgaggaatt ttagcattcc aaaccacctt gcccggcaca cacggactca
     8941 cacaggggag aagcccttcc agtgtagaat ttgcatgcgg aacttcagcc agtcagccca
     9001 cttgaaaaga cacctgcgga cccatactgg cgaaaagccc tttcagtgcc ggatctgcat
     9061 gcgcaacttc tcccaggacg tttcccttgt caggcacctg aagacacatc ttcggcagaa
     9121 agatggagag agaccattcc agtgtcggat ttgtatgagg aactttagtt ctgcacaggc
     9181 tttggctagg catacccgga cccacacagg tgagaagcca ttccagtgtc gcatctgcat
     9241 gcggaatttc tcccagggag gcaacctcac taggcacctg cggactcata caggggagaa
     9301 accttttcag tgtaggatct gcatgcgcaa tttttcacag catccaaatt tgacgcgtca
     9361 cctgaaaacc cacctgagag gatcctaaaa gcttgatggg ggatcctagg agcaggtttc
     9421 cccaatgaca caaaacgtgc aacttgaaac tccgcctggt ctttccaggt ctagaggggt
     9481 aacactttgt actgcgtttg gctccacgct cgatccactg gcgagtgtta gtaacagcac
     9541 tgttgcttcg tagcggagca tgacggccgt gggaactcct ccttggtaac aaggacccac
     9601 ggggccaaaa gccacgccca cacgggcccg tcatgtgtgc aaccccagca cggcgacttt
     9661 actgcgaaac ccactttaaa gtgacattga aactggtacc cacacactgg tgacaggcta
     9721 aggatgccct tcaggtaccc cgaggtaaca cgcgacactc gggatctgag aaggggactg
     9781 gggcttctat aaaagcgctc ggtttaaaaa gcttctatgc ctgaataggt gaccggaggt
     9841 cggcaccttt cctttgcaat tactgaccct atgaatacac tgactgtttg acaattaatc
     9901 atcggcatag tatatcggca tagtataata cgactcacta taggagggcc accatgattg
     9961 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
    10021 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
    10081 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
    10141 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
    10201 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
    10261 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
    10321 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
    10381 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
    10441 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
    10501 atctcgtcgt gacacatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
    10561 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
    10621 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
    10681 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
    10741 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgaattcgc taggattatc
    10801 cctaatacct gccaccccac tcttaatcag tggtggaaga acggtctcag aactgtttgt
    10861 ttcaattggc catttaagtt tagtagtaaa agactggtta atgataacaa tgcatcgtaa
    10921 aaccttcaga aggaaaggag aatgttttgt ggaccacttt ggttttcttt tttgcgtgtg
    10981 gcagttttaa gttattagtt tttaaaatca gtacttttta atggaaacaa cttgaccaaa
    11041 aatttgtcac agaattttga gacccattaa aaaagttaaa tgagaaacct gtgtgttcct
    11101 ttggtcaaca ccgagacatt taggtgaaag acatctaatt ctggttttac gaatctggaa
    11161 acttcttgaa aatgtaattc ttgagttaac acttctgggt ggagaatagg gttgttttcc
    11221 ccccacataa ttggaagggg aaggaatatc atttaaagct atgggagggt tgctttgatt
    11281 acaacactgg agagaaatgc agcatgttgc tgattgcctg tcactaaaac aggccaaaaa
    11341 ctgagtcctt gggttgcata gaaagctg
//
