LOCUS       sequence-121090-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Maier V, Jolicoeur C, Rayburn H, Takegahara N, Kumanogoh A, Kikutani
            H, Tessier-Lavigne M, Wurst W, Friedel RH
  TITLE     Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar
            development.
  JOURNAL   Mol Cell Neurosci. 2011 Feb;46(2):419-31. doi: 
            10.1016/j.mcn.2010.11.005. Epub 2010 Nov 29.
  PUBMED    21122816
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.mcn.2010.11.005"; journalName: "Mol Cell Neurosci"; date:
            "2011-02"; volume: "46"; issue: "2"; pages: "419-31"
COMMENT     Sequence Label: pmPB3-VSV
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             293..325
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgcatgcct gcaggtcagc ttgatttcac
      121 ccaggcatct ggtccagagg gatccgccag cccgtccagc agcaccatgt gggtgaccaa
      181 actcctgcca gccctgctgc tgcagcatgt cctcctgcat ctcctcctgc tccccatcgc
      241 catcccctat gcagagggac aaaggaaaag aagaaataca attcatgaat tctatacaga
      301 catagagatg aaccgacttg gaaagggcgg agtcgaccat cgcttctctg taccaaatac
      361 cagcttcaac cacctggtgc tggcaccaga tcagggcaaa ctctacgtgg gtgcagtgaa
      421 tcacctcttt cagctcagcc ctgagctgaa gatggagtct gtggctgtca ctggccctgt
      481 cattgacagt cctgattgtg tgcctttccg tgacctggct gagtgcccac aggcccagct
      541 cactgacaat gccaaccagc tcttgttggt gagcagcagg acacaggaac tggtggcttg
      601 tgggcaagtg aagcagggtg tatgtgagaa gcggcgcctt ggggatgtga ctcaagtgtt
      661 gtaccaggct gaggaccctg gtgatgggca gtttgtggct gccaataccc tgggagtgac
      721 cacagtaggc ctagtggtgc ccttgccagg ccgagacctc ctgctagtgg ccagaggcct
      781 ggcaggcaag ctgtcagcag gagtgccacc cctaactgtg cgccagctgg ctgggccaca
      841 gcctttctcc agtgaaggac tgggaagact tgtggtcggt gacttttcag actacaacaa
      901 tagctatgta ggagcttttt ctgatgctca ctctgcctac tttgtgttcc gtcgccgtgg
      961 ggctcgggcc cagactgagt atcgctccta tgtagctcgt gtctgcctca gagatgtcaa
     1021 cctttattcc tatgtggaaa tgccacttac ctgccatggc cagggcctta tccaggctgc
     1081 cttccttact ccagaca
//
