LOCUS       sequence-121091-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-q construct contains internal insertions of YFP
            and the EE epitope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: G protein alpha-q-YFP
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..229
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(806..825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(807..825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
       61 agcacatcag gccgtagccg aaggtggtca cgagggtggg ccagggcacg ggcagcttgc
      121 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      181 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      241 ccccggtgaa cagctcctcg cccttgctca ccatggatcc acctccacca gaaaaagcag
      301 acaccttctc cacatcaacc tctcgaacca attgtgcatg agccttattg tgttcatact
      361 tgtatgggat cttgagtgtg tccatcgctc tgatcatggc ctgcatggcc gtgaagatgt
      421 tctgatacac cagcttggtg aagccgcgct tgtcttcgtc agagtagccc gacccgtgga
      481 tgatcctcat ctgcttgatg aaggtgctct tgccactctc ccctgtcccc agcagcagca
      541 gcttgagttc ccggcgggcg tcgcgcttgt ccctgcgcag ctgccgctcg atctcgtcgt
      601 tgatcctccg ggcttccttg gcctcctcgc tcaggcagca cgccatgatg gactccagag
      661 tcattcttcc gaagtgcctc gctgcagccc cgccggcgcg gagcttcacc gcggtggcgg
      721 ccgccagtgt gatggatatc tgcagaattc cagcacactg gcggccgtta ctagtggatc
      781 cgagctcggt accaagcttg ggtctcccta tagtgagtcg tattaatttc ga
//
