LOCUS       sequence-121092-        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  middle entry plasmid with dimerizable extracellular domain fused to 
            Danio Flt4 cytoplasmic domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pME-Fv2E-flt4-ns
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             112..153
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             160..480
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             487..807
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag caggctggac catggggagt
      121 agcaagagca agcctaagga ccccagccag cgctctagag gcgtccaagt cgaaaccatt
      181 agtcccggcg atggcagaac atttcctaaa aggggacaaa catgtgtcgt ccattataca
      241 ggcatgttgg aggacggcaa aaaggtggac agtagtagag atcgcaataa acctttcaaa
      301 ttcatgttgg gaaaacaaga agtcattagg ggatgggagg agggcgtggc tcaaatgtcc
      361 gtcggccaac gcgctaagct caccatcagc cccgactacg catacggcgc taccggacat
      421 cccggaatta ttccccctca cgctaccttg gtgtttgacg tcgaactgtt gaagctcgag
      481 actagaggag tgcaggtgga gactatctcc ccaggagacg ggcgcacctt ccccaagcgc
      541 ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa agttgattcc
      601 tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc
      661 tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca
      721 gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc
      781 ttcgatgtgg agcttctaaa actggaaact agagtgaatg tcaagcgagt gaacccagct
      841 gatatcaaga caggctatct gtccatcatc atggacccgg gggaagtgcc gctggaggag
      901 cagtgtgaat acctgccgta cgactccagc cagtgggaga tttccagaga cagactgcgc
      961 ctcgggaagg tgctcggcca tggagctttc gggaaggtga ttgaagcctc aatctttggt
     1021 catgacaaga aatcctctgc aaacacagtg gctgtcaaga tgttgaaaga gggagcaaca
     1081 gccagcgagc acaaagccct gatgtcagag ctgaagatcc tcattcatat tggaaacc
//
