LOCUS       sequence-121100-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses 
            tagRFP via P2A cleavage site. EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.EFS.tRFP
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             126..146
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
       61 aacacaggtg tcgtgacgcg ggatccgcca ccatggatta caaagacgat gacgataaga
      121 tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt
      181 acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
      241 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
      301 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
      361 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
      421 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
      481 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
      541 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
      601 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
      661 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
      721 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
      781 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
      841 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga
      901 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
      961 agctgagcaa ggacac
//
