LOCUS       sequence-121101-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses 
            tagRFP via P2A cleavage site. EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.EFS.tRFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..93)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(109..156)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gttctcctta atcagctctt cgcccttaga caccatgggg ccggggttct cctccacgtc
       61 gccggcctgc ttcagcaggc tgaagttggt ggcgccgctg ccgctagctt tctttttctt
      121 agcttgacca gctttcttag tagcagcagg acgcttgtcg cctcccagct gagacaggtc
      181 gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt ccagcacctc
      241 tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga aggcggcagg
      301 ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct ctctgatggg
      361 cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt cggccaggat
      421 cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt gcttgtgctg
      481 ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct tctcatagtg
      541 gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt ttcccttctg
      601 cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga acagggagta
      661 cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg cttccagaaa
      721 gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca gcagctcttt
      781 cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca gcacagaata
      841 ggccacggtg gggctgtcga agccgccgta cttctta
//
