LOCUS       sequence-121104-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry clone with 6xmyc nuclear localized tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: p3E-2a-6MTnlstdTomato
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..177)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             189..245
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             273..302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             312..341
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             351..380
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             390..419
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             429..458
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             492..521
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             546..566
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(700..718)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cagctctaga gctcgaattc tacaggtcac taataccatc taagtagttg gttcatagtg
       61 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      121 atatattgat atttatatca ttttacgttt ctcgttcaac tttcttgtac aaagtgggag
      181 gatccggagc cacgaacttc tctctgttaa agcaagcagg agacgtggaa gaaaaccccg
      241 gtcctgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct gaagaggact
      301 tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagcaaaagc
      361 tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga
      421 atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag agcttgggcg
      481 acctcaccat ggagcaaaag ctcatttctg aagaggactt gaatttagag atctcgaatt
      541 ccatgcccaa gaagaagagg aaggtgatgg tgagcaaggg cgaggaggtc atcaaagagt
      601 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
      661 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      721 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
      781 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
      841 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
      901 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcac
//
