LOCUS       sequence-121109-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ELP[V-150] in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pET-24a-ELP[V-150]
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..57)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     53..71
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(124..141)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcctttcagc aaaaaacccn tcaagacccg tttagaggcc ccaaggggtt atgctagtta
       61 ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc
      121 tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc ttgtcgacgg agctcgaatt
      181 cggatcctga agattatcac ggccagggaa ctccaacacc tacaccaggc actccaaccc
      241 caggaactcc cacccctgga acccctacac ctggaacacc taccccaggg acgccgaccc
      301 cgggcacgcc cacacctggt acgccaacac ctgggacccc tactcctgga actcctaccc
      361 ccggaactcc aactccagga acaccaactc cgggtactcc aacaccaggt actccgacac
      421 cagggactcc gactccaggc accccaactc ctggcacacc tacgcccggt acccctaccc
      481 ctggtacacc gactcctggg actcccacac caggaacccc cactcctggt actcctactc
      541 ccggtactcc cactccaggt acaccaacac cggggactcc tacacccggg actccaacgc
      601 cgggaacacc cacgccaggt accccgacgc ctggaacgcc tactccaggg acacctaccc
      661 caggaacacc tacaccaggc actccaaccc caggaactcc cacccctgga acccctacac
      721 ctggaaca
//
