LOCUS       sequence-121111-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: p3EHA
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..175)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             178..204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(209..304)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(341..360)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(342..360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(365..381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(365..381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(483..502)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(561..580)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gctctagagc tcgaattcta caggtcacta ataccatcta agtagttggt tcatagtgac
       61 tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      121 atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa agtggactat
      181 ccgtacgacg taccagacta cgcataacca actttattat acaaagttgg cattataaaa
      241 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
      301 atttggagct ccatggtagc gttaacgcgg ccgcgatatc ccctatagtg agtcgtatta
      361 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
      421 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
      481 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
      541 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
      601 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
      661 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
      721 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
      781 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
      841 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
      901 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
      961 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     1021 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtg
//
