LOCUS       sequence-121115-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of mCherry fused FRB to the mitochondrial 
            innermembrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Ichim G, Lopez J, Ahmed SU, Muthalagu N, Giampazolias E, Delgado ME,
            Haller M, Riley JS, Mason SM, Athineos D, Parsons MJ, van de Kooij 
            B, Bouchier-Hayes L, Chalmers AJ, Rooswinkel RW, Oberst A, Blyth K, 
            Rehm M, Murphy DJ, Tait SW
  TITLE     Limited mitochondrial permeabilization causes DNA damage and genomic
            instability in the absence of cell death.
  JOURNAL   Mol Cell. 2015 Mar 5;57(5):860-72. doi: 
            10.1016/j.molcel.2015.01.018. Epub 2015 Feb 19.
  PUBMED    25702873
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.018"; journalName: "Mol Cell"; date: 
            "2015-03-5- 5"; volume: "57"; issue: "5"; pages: "860-72"
COMMENT     Sequence Label: pcDNA3 AIF 1-90 mCherry FRB
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..306)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     primer_bind     complement(603..622)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gggccctcta gatgcatgct cgagtcactt tgagattcgt cggaacacat gataatagag
       61 gtcccaggct tggaggaggt ccttgacatt ccctgatttc atgtacttcc tgcaccactc
      121 ttgggcctcc attaaatctc gaccataggc ctgattaaag gatgtttcct tcagagtctg
      181 ggggccccgt tccatcatag catgcaaggg ctccagcacc tcaaacatgc ctttcacgtt
      241 cctttcccca aagtacaaac gagatgcctc ttccaggcct tcatgccaca tctcatgcca
      301 gaggatcccg ccacctccgg cggccgcctt gtacagctcg tccatgccgc cggtggagtg
      361 gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat
      421 gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta
      481 ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat
      541 ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct cccagcccat
      601 ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt
      661 gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca cgccgccgtc
      721 ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct tcaagtagtc
//
