LOCUS       sequence-121122-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of mCherry fused FRB to the mitochondrial 
            innermembrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Ichim G, Lopez J, Ahmed SU, Muthalagu N, Giampazolias E, Delgado ME,
            Haller M, Riley JS, Mason SM, Athineos D, Parsons MJ, van de Kooij 
            B, Bouchier-Hayes L, Chalmers AJ, Rooswinkel RW, Oberst A, Blyth K, 
            Rehm M, Murphy DJ, Tait SW
  TITLE     Limited mitochondrial permeabilization causes DNA damage and genomic
            instability in the absence of cell death.
  JOURNAL   Mol Cell. 2015 Mar 5;57(5):860-72. doi: 
            10.1016/j.molcel.2015.01.018. Epub 2015 Feb 19.
  PUBMED    25702873
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.018"; journalName: "Mol Cell"; date: 
            "2015-03-5- 5"; volume: "57"; issue: "5"; pages: "860-72"
COMMENT     Sequence Label: pcDNA3 AIF 1-90 mCherry FRB
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             337..366
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(515..533)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     783..802
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttatgttcc ggtgtggagg cctggcggcg ggtgctttga agcagaagct ggtgcccttg
      121 gtgcggaccg tgtgcgtccg aagcccgagg cagaggaacc ggctcccagg caacttgttc
      181 cagcgatggc atgttcctct agaactccag atgacaagac aaatggctag ctctggtgca
      241 tcagggggca aaatcgataa ttctgtgtta gtccttattg tgggcttatc aacagtagga
      301 gctggtgcct atgcctacaa gactatgaaa gagatggaac aaaaacttat ttctgaagaa
      361 gatctgggta ccgtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      421 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      481 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      541 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      601 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      661 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      721 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      781 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      841 gaggacggcg
//
