LOCUS       sequence-121127-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_307
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(110..234)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             242..283
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(387..407)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
      121 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      181 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatat
      241 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtac
      301 cggttaagtc gacaatcaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
      361 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
      421 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
      481 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
      541 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
      601 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
      661 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgt
//
