LOCUS       sequence-121130-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PI driving expression dimeric LacI, the Lactose inducible 
            repressor. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[LacI-L]
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     82..100
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        165..242
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(262..281)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gttccgcgca catttccccg aaaagtgcca cctgacgtgc tagcctaaga aaccattatt
       61 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctcgac
      121 aattcgcgcg cgaaggcgaa gcggcatgca tttacgttgg taccgacacc atcgaatggc
      181 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
      241 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
      301 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
      361 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
      421 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
      481 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
      541 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
      601 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
      661 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
      721 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
      781 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
      841 gctggcataa atatctcact cgcaatcaaa tt
//
