LOCUS       sequence-121132-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 PC
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..39
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacgagctat acaagctgga ggtgctcttc cagggtccga caagtttgta caaaaaagtt
       61 ggcatgctga agttccgaac agtccatggg ggcctgaggc tcctgggaat ccgccgaacc
      121 tccaccgccc ccgctgcctc cccaaatgtc cggcgcctgg agtataagcc catcaagaaa
      181 gtcatggtgg ccaacagagg tgagattgcc atccgtgtgt tccgggcctg cacggagctg
      241 ggcatccgca ccgtagccat ctactctgag caggacacgg gccagatgca ccggcagaaa
      301 gcagatgaag cctatctcat cggccgcggc ctggcccccg tgcaggccta cctgcacatc
      361 ccagacatca tcaaggtggc caaggagaac aacgtagatg cagtgcaccc tggctacggg
      421 ttcctctctg agcgagcgga cttcgcccag gcctgccagg atgcaggggt ccggtttatt
      481 gggccaagcc cagaagtggt ccgcaagatg ggagacaagg tggaggcccg ggccatcgcc
      541 attgctgcgg gtgttcccgt tgtccctggc acagatgccc ccatcacgtc cctgcatgag
      601 gcccacgagt tctccaacac ctacggcttc cccatcatct tcaaggcggc ctatgggggt
      661 ggagggcgtg gcatgagggt ggtgcacagc tacgaggagc tggaggagaa ttacacccgg
      721 gcctactcag aggctctggc cgcctttggg aatggggcgc tgtttgtgga gaagttcatc
      781 gagaagccac ggcacatcga ggtgcagatc ttgggggacc agtatgggaa catcctgcac
      841 ctgtacgagc gagactgctc catccagcgg cggcaccaga aggtggtcga gattgcccc
//
