LOCUS       sequence-121133-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 PC
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagttgg gcactcgatc tccaggatga ggtcgtcacc ttccagtgtc atgtccttgg
      241 tcacatgaac cttgcggaca gtaccctcca tgggtgaggt caccacagtc tccatcttca
      301 tggcactgag cacacacagg ggctggccct tggccacctt ggcccctgcc accactttga
      361 tgtctatcac cttcccaggc atgggcgccc cgatctggcc cttcacgtcc tttagggcct
      421 tggggtggaa gtgcatctcc ttcatggcct gggtgtcctt gaccaagatg gaccgcagct
      481 gcccattgag ctcaaagaag acctgcctct ggccggcccg gttcaggtcg ctcacggcca
      541 gggctttgat gtgcagcgtc ttgccccgct ccagctccac ctcaaactcc tctgcgatct
      601 tgggtccctg caggaagagg cgagtattca ggctatccag ggggccaaag gtggcagtga
      661 agtccttgaa gtgggcaaac acatcggggt acatagctgc tgagagcaca tcttccggcg
      721 tcacctcctc cccatgccgg tctaccagct ccttctccag tgcctgcaga tccagg
//
