LOCUS       sequence-121172-        3417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replacement for 26033 776 pME-mir-126.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND
  TITLE     MicroRNA-mediated integration of haemodynamics and Vegf signalling 
            during angiogenesis.
  JOURNAL   Nature. 2010 Apr 22. 464(7292):1196-200.
  PUBMED    20364122
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Apr 22. 464(7292):1196-200."
COMMENT     Sequence Label: pME-mir126new
FEATURES             Location/Qualifiers
     source          1..3417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1542..1641)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1658..1677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1659..1677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1682..1698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1682..1698)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1800..1819)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1811..2620
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1878..1897)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2767..3355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3256..3275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctct cgagtataaa ttctccaacc aaagcgtttt tcttcctctt tctgttacct
      721 ggcaaagggg agcagcagct gaggagtgat ctctcaatct tggtgagtac tataggctag
      781 gcctctgtgg taccattcta catgtgttga ttttctgtat tttagtgaat tctgtgatcg
      841 ataactttaa tgcgtatatt gaggcttttc ttcacggtgg cggatgtttt tatgacattt
      901 ttacgcggcg gtttgttatc cggcgagctt ttgtcagtta ttttatgttc ggttttctat
      961 gtggcttgta tgtctttctt aaatgtttta ttttaataag tagtgcgttt tgagccattt
     1021 taactgcttc acagtccatt attacttttg gtacgcgcta ggccagactc aaactcgtac
     1081 cgtgagtaat aatgcactgt ggcagtgggt ttaaagttga ctttgttgtc tctttatatt
     1141 tctgctgttt ccgttatttt gtgtgtgaaa tgagcaccgg gaaaagaaca aaggcacaag
     1201 cggtttgaag tcacgggtcc gttaaaagcc tatcacacat tcccgcatcc agcaactgtt
     1261 acaagtggcc ggagagccag atgtctgcct cagtttggat tccgttcaga catttctgtt
     1321 ttttttaatc atcctgtaac ttaacaccgt cttactctaa agaaacacgt gtcgcagctg
     1381 ccttatggtg aactaggccg gttcaaactc tgggccatct tgtcacttgt gttacgaaat
     1441 caccgctcaa aagttttaat aatgagaaat atgtattttt gtggtccatc attaactttc
     1501 acattttatc ttggtccgca gaaacttagt cgacggtatc gacccagctt tcttgtacaa
     1561 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1621 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     1681 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     1741 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     1801 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     1861 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     1921 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     1981 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     2041 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     2101 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     2161 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     2221 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     2281 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     2341 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     2401 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     2461 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     2521 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     2581 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     2641 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     2701 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2761 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2821 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2881 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2941 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3001 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3061 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
