LOCUS       sequence-121173-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replacement for 26033 776 pME-mir-126.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND
  TITLE     MicroRNA-mediated integration of haemodynamics and Vegf signalling 
            during angiogenesis.
  JOURNAL   Nature. 2010 Apr 22. 464(7292):1196-200.
  PUBMED    20364122
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Apr 22. 464(7292):1196-200."
COMMENT     Sequence Label: pME-mir126new
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     164..180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     164..180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     185..204
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        185..203
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    221..320
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctcngtggaa tttaatcgcg gcctcgacgt ttcccgttga atatggctca taacacccct
       61 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
      121 tgcaatgtaa catcagagat tttgagacac gggccagagc tgccaggaaa cagctatgac
      181 catgtaatac gactcactat aggggatatc agctggatgg caaataatga ttttattttg
      241 actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttctta taatgccaac
      301 tttgtacaag aaagctgggt cgataccgtc gactaagttt ctgcggacca agataaaatg
      361 tgaaagttaa tgatggacca caaaaataca tatttctcat tattaaaact tttgagcggt
      421 gatttcgtaa cacaagtgac aagatggccc agagtttgaa ccggcctagt tcaccataag
      481 gcagctgcga cacgtgtttc tttagagtaa gacggtgtta agttacagga tgattaaaaa
      541 aaacagaaat gtctgaacgg aatccaaact gaggcagaca tctggctctc cggccacttg
      601 taacagttgc tggatgcggg aatgtgtgat aggcttttaa cggacccgtg acttcaaacc
      661 gcttgtgcct ttgttctttt cccggtgctc atttcacaca caaaataacg gaaacagcag
      721 aaatataaag agacaacaaa gtcaacttta aacccactgc cacagtgcat tattactcac
      781 ggtacgagtt tgagtctggc ctagcgcgta ccaaaagtaa taatggactg tgaagcagtt
      841 aaaatggctc aaaacgcact acttattaaa ataaaacatt taagaaagac atacaagcca
      901 catagaaaac cgaacataaa ataactgaca aaagctcgcc ggataacaaa ccgccgcgta
      961 aaaatgtcat aaaaacatcc gccaccgtga agaaaagcct caatatacgc attaaagtta
     1021 tcgatcacag aattcactaa aatacagaaa atcaacacat gtagaatggt accaca
//
