LOCUS       sequence-121179-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OR2-OR1-Pr1 promoter expressing T7 RNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
  TITLE     Gene circuit performance characterization and resource usage in a 
            cell-free ""breadboard"".
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. 
            Epub 2014 Apr 7.
  PUBMED    24670245
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400203p";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "416-25"
COMMENT     Sequence Label: Pr1-T7RNAP
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             156..178
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tgctgggtgg gctaacgata tccgcctgat gcgtgaacgt gacggacgta accaccgcga
       61 catgtgtgtg ctgttccgct gggcatgctg agctaacacc gtgcgtgtag acaattttac
      121 ctctggcggt gataatggtt gcagctagca ataattttgt ttaactttaa gaaggagata
      181 taccatgaac acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat
      241 cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac agttggccct
      301 tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact
      361 taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc
      421 taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc gcggcaagcg
      481 cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt acatcaccat
      541 taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag
      601 cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc
      661 taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa
      721 gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac tcggtggcga
      781 ggcgtggtct t
//
