LOCUS       sequence-121183-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
  TITLE     Gene circuit performance characterization and resource usage in a 
            cell-free ""breadboard"".
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. 
            Epub 2014 Apr 7.
  PUBMED    24670245
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400203p";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "416-25"
COMMENT     Sequence Label: Pr-deCFP-MGapt
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             156..178
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(215..236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(476..495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgctgggtgg gctaacgata tccgcctgat gcgtgaacgt gacggacgta accaccgcga
       61 catgtgtgtg ctgttccgct gggcatgctg agctaacacc gtgcgtgttg acaattttac
      121 ctctggcggt gataatggtt gcagctagca ataattttgt ttaactttaa gaaggagata
      181 taccatggag cttttcactg gcgttgttcc catcctggtc gagctggacg gcgacgtaaa
      241 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      301 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      361 cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      481 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      601 caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
      661 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      721 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      781 ccagtccgcc
//
