LOCUS       sequence-121185-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 promoter expressing deGFP-MG aptamer fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
  TITLE     Gene circuit performance characterization and resource usage in a 
            cell-free ""breadboard"".
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. 
            Epub 2014 Apr 7.
  PUBMED    24670245
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400203p";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "416-25"
COMMENT     Sequence Label: PT7-deGFP-MGapt
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     297..316
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     556..577
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             614..636
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(667..686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
       61 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      121 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      181 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      241 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      301 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      361 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
      421 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      481 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
      541 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggaacaacg ccagtgaaaa
      601 gctccatggt atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg
      661 tggtctccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
      721 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc cta
//
