LOCUS       sequence-121186-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pr promoter expressing deGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
  TITLE     Gene circuit performance characterization and resource usage in a 
            cell-free ""breadboard"".
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. 
            Epub 2014 Apr 7.
  PUBMED    24670245
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400203p";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "416-25"
COMMENT     Sequence Label: Pr-deGFP
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             158..180
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(217..238)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(478..497)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttgctgggt gggctaacga tatccgcctg atgcgtgaac gtgacggacg taaccaccgc
       61 gacatgtgtg tgctgttccg ctgggcatgc tgagctaaca ccgtgcgtgt tgacaatttt
      121 acctctggcg gtgataatgg ttgcagctag caataatttt gtttaacttt aagaaggaga
      181 tataccatgg agcttttcac tggcgttgtt cccatcctgg tcgagctgga cggcgacgta
      241 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      301 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      361 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      421 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      481 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      541 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      601 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
      661 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
      721 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      781 acccagtccg c
//
