LOCUS       sequence-121189-       16542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducbile color change and cancer regulation: FlpO recombinase 
            dependent expression of KrasG12D cMyc and SV40 large T antigen with 
            doxycycline inducible downregulation through tetKRAB system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16542)
  AUTHORS   Callesen MM, Arnadottir SS, Lyskjaer I, Orntoft MW, Hoyer S, 
            Dagnaes-Hansen F, Liu Y, Li R, Callesen H, Rasmussen MH, Berthelsen 
            MF, Thomsen MK, Schweiger PJ, Jensen KB, Laurberg S, Orntoft TF, 
            Elverlov-Jakobsen JE, Andersen CL
  TITLE     A genetically inducible porcine model of intestinal cancer.
  JOURNAL   Mol Oncol. 2017 Nov;11(11):1616-1629. doi: 10.1002/1878-0261.12136. 
            Epub 2017 Oct 10.
  PUBMED    28881081
REFERENCE   2  (bases 1 to 16542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1878-0261.12136"; journalName: "Mol Oncol"; date: 
            "2017-11"; volume: "11"; issue: "11"; pages: "1616-1629"
COMMENT     Sequence Label: 
            LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-S
            V40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
FEATURES             Location/Qualifiers
     source          1..16542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    698..731
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(754..772,773..795,796..814,815..837,838..856,857..879,
                     880..898,899..921,922..940,941..963,964..982,983..1005,
                     1006..1024)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     enhancer        1100..1403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1406..1681
                     /label=chicken beta-actin promoter
     primer_bind     2616..2638
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2703..2719
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2729..2748
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2729..2747
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2862..2909
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      2910..2919
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2916..3620
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     misc_feature    3621..3686
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3714..3773
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3780..4379
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3780..3799)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4276..4296
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4426..4650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5057..5104
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    5119..5150
                     /label=rox
                     /bound_moiety="Dre recombinase"
     regulatory      5161..5170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5167..5169,5170..5172,5173..5883)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5212..5233)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5473..5492)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5820..5841
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5893..5958
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(5959..5988,5989..5991,5992..6519)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="TEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             5959..6519
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="TEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
                     SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIR
                     KHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             6529..6582
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6580..7893
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="PLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPS
                     EDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTEL
                     LGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNP
                     ARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSL
                     LSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSP
                     SAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRK
                     CTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKAT
                     AYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             7909..7965
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7966..10089
                     /codon_start=1
                     /product="large T antigen from SV40 (simian virus 40)"
                     /label=large T antigen
                     /translation="DKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKG
                     GDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFC
                     SEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTT
                     KEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLIC
                     KGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKC
                     DDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAV
                     DTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPK
                     MDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNF
                     ELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRT
                     QIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLL
                     MLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDD
                     EDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTC
                     FKKPPTPPPEPET"
     misc_feature    10120..10686
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    10137..10688
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10281..10298)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10508..10527
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11312..11332
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11435..11629
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     misc_feature    11771..12359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11824..11844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12242..12253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(12497..12515,12516..12538,12539..12557,12558..12580,
                     12581..12599,12600..12622,12623..12641,12642..12664,
                     12665..12683,12684..12706,12707..12725,12726..12748,
                     12749..12767)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             12806..12986
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    13099..13220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    13227..13258
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    13371..13492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    13564..13788
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(13805..13838)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(14321..14337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14321..14337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14334..14356)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14345..14361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14369..14375,14376..14393,14394..14399))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14414..14435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14552..14569)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14723..15311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14803..14822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15482..16273,16274..16342))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16105..16124
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16343..16447)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16515..16533
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cagatctgat atctctagag
      421 aagtcgacgt ttaaagtacc ctacagttga agtcggaagt ttacatacac ttaagttgga
      481 gtcattaaaa ctcgtttttc aactactcca caaatttctt gttaacaaac aatagttttg
      541 gcaagtcagt taggacatct actttgtgca tgacacaagt catttttcca acaattgttt
      601 acagacagat tatttcactt ataattcact gtatcacaat tccagtgggt cagaagttta
      661 catacactaa gttgactgtg cctttaaaca gtcgcgaata acttcgtata atgtatgcta
      721 tacgaagtta tggcgcgccc tcgagtttac cactccctat cagtgataga gaaaagtgaa
      781 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      841 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      901 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      961 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     1021 gagaaaagtg aaagtcgact agttattaat agtaatcaat tacggggtca ttagttcata
     1081 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1141 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1201 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1261 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1321 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1381 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
     1441 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     1501 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     1561 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     1621 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     1681 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
     1741 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     1801 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
     1861 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
     1921 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
     1981 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     2041 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     2101 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     2161 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     2221 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     2281 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     2341 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     2401 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     2461 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     2521 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     2581 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     2641 cggctctagt aacggccgcc agtgtgctgg aattcgccct tcaagcttcc agtcacgacg
     2701 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct
     2761 cgcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     2821 ggttgttgtg ctgtctcatc attttggcaa agattaatta agaagttcct attccgaagt
     2881 tcctattctc tagaaagtat aggaacttcg ccaccatggt gggtgaggat agcgtgctga
     2941 tcaccgagaa catgcacatg aaactgtaca tggagggcac cgtgaacgac caccacttca
     3001 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg aagatcaagg
     3061 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
     3121 gcagcaaaac ctttatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
     3181 ctgagggctt cacatgggag aggatcacca catacgaaga cgggggcgtg ctgaccgcta
     3241 cccaggacac cagcctccag aacggctgcc tcatctacaa cgtcaagatc aacggggtga
     3301 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gccagcaccg
     3361 agatgctgta ccccgctgac agcggcctga gaggccatag ccagatggcc ctgaagctcg
     3421 tgggcggggg ctacctgcac tgctccctca agaccacata cagatccaag aaacccgcta
     3481 agaacctcaa gatgcccggc ttctacttcg tggacaggag actggaaaga atcaaggagg
     3541 ccgacaaaga gacctacgtc gagcagcacg agatggctgt ggccaggtac tgcgacctgc
     3601 ctagcaaact ggggcacagc tccggactca gatctcgagc tcaagcttcg aattctgcag
     3661 tcgacggtac cgcgggcccg ggatccaccg gatctagacc taggggaagc ggacagtgta
     3721 ctaattatgc tctcttgaaa ttggctggag atgttgagag caaccctgga cctaccggta
     3781 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     3841 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     3901 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     3961 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4021 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     4081 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     4141 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     4201 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     4261 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     4321 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     4381 tcgatgactc tagatcataa tcagcccggg ggtgatcagc ctcgactgtg ccttctagtt
     4441 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4501 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4561 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4621 ggcatgctgg ggatgcggtg ggctctatgg aaccagctgg ttgtttattg cagcttataa
     4681 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4741 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcttgtttat
     4801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4921 gatcttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5041 ttatcatgtc tggatcgaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     5101 cttcctcatg cgcataacta actttaaata atgccaatta tttaaagtta ttgcgatcgc
     5161 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     5221 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     5281 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     5341 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
     5401 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5461 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5521 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5581 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     5641 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     5701 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5761 aaccactacc tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     5821 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5881 aagggaagcg gagtgaaaca gactttgaat tttgaccttc tcaagttggc gggagacgtg
     5941 gagtccaacc ctggacctac tgaatataaa cttgtggtag ttggagctga tggcgtaggc
     6001 aagagtgcct tgacgataca gctaattcag aatcattttg tggacgaata tgatccaaca
     6061 atagaggatt cctacaggaa gcaagtagta attgatggag aaacctgtct cttggatatt
     6121 ctcgacacag caggtcaaga ggagtacagt gcaatgaggg accagtacat gaggactggg
     6181 gagggctttc tttgtgtatt tgccataaat aatactaaat catttgaaga tattcaccat
     6241 tatagagaac aaattaaaag agttaaggac tctgaagatg tacctatggt cctagtagga
     6301 aataaatgtg atttgccttc tagaacagta gacacaaaac aggctcagga cttagcaaga
     6361 agttatggaa ttccttttat tgaaacatca gcaaagacaa gacagggtgt tgatgatgcc
     6421 ttctatacat tagttcgaga aattcgaaaa cataaagaaa agatgagcaa agatggtaaa
     6481 aagaagaaaa agaagtcaaa gacaaagtgt gtaattatgg gaagcggaga gggcagagga
     6541 agtctgctaa catgcggtga cgtcgaggag aatcctggac ctctcaacgt tagcttcacc
     6601 aacaggaact atgacctcga ctacgactcg gtgcagccgt atttctactg cgacgaggag
     6661 gagaacttct accagcagca gcagcagagc gagctgcagc ccccggcgcc cagcgaggat
     6721 atctggaaga aattcgagct gctgcccacc ccgcccctgt cccctagccg ccgctccggg
     6781 ctctgctcgc cctcctacgt tgcggtcaca cccttctccc ttcggggaga caacgacggc
     6841 ggtggcggga gcttctccac ggccgaccag ctggagatgg tgaccgagct gctgggagga
     6901 gacatggtga accagagttt catctgcgac ccggacgacg agaccttcat caaaaacatc
     6961 atcatccagg actgtatgtg gagcggcttc tcggccgccg ccaagctcgt ctcagagaag
     7021 ctggcctcct accaggctgc gcgcaaagac agcggcagcc cgaaccccgc ccgcggccac
     7081 agcgtctgct ccacctccag cttgtacctg caggatctga gcgccgccgc ctcagagtgc
     7141 atcgacccct cggtggtctt cccctaccct ctcaacgaca gcagctcgcc caagtcctgc
     7201 gcctcgcaag actccagcgc cttctctccg tcctcggatt ctctgctctc ctcgacggag
     7261 tcctccccgc agggcagccc cgagcccctg gtgctccatg aggagacacc gcccaccacc
     7321 agcagcgact ctgaggagga acaagaagat gaggaagaaa tcgatgttgt ttctgtggaa
     7381 aagaggcagg ctcctggcaa aaggtcagag tctggatcac cttctgctgg aggccacagc
     7441 aaacctcctc acagcccact ggtcctcaag aggtgccacg tctccacaca tcagcacaac
     7501 tacgcagcgc ctccctccac tcggaaggac tatcctgctg ccaagagggt caagttggac
     7561 agtgtcagag tcctgagaca gatcagcaac aaccgaaaat gcaccagccc caggtcctcg
     7621 gacaccgagg agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac
     7681 gagctaaaac ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa
     7741 aaggccccca aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca
     7801 gaggagcaaa agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa
     7861 cacaaacttg aacagctacg gaactcttgt gcggtatacg gaagcggagc tactaacttc
     7921 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctgataa agttttaaac
     7981 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat
     8041 attcctctga tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga
     8101 ggagatgaag aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta
     8161 aaatatgctc atcaacctga ctttggaggc ttctgggatg caactgagat tccaacctat
     8221 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     8281 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     8341 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     8401 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     8461 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     8521 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     8581 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     8641 aataaggaat atttgatgta tagtgccttg actagagatc cattttctgt tattgaggaa
     8701 agtttgccag gtgggttaaa ggagcatgat tttaatccag aagaagcaga ggaaactaaa
     8761 caagtgtcct ggaagcttgt aacagagtat gcaatggaaa caaaatgtga tgatgtgttg
     8821 ttattgcttg ggatgtactt ggaatttcag tacagttttg aaatgtgttt aaaatgtatt
     8881 aaaaaagaac agcccagcca ctataagtac catgaaaagc attatgcaaa tgctgctata
     8941 tttgctgaca gcaaaaacca aaaaaccata tgccaacagg ctgttgatac tgttttagct
     9001 aaaaagcggg ttgatagcct acaattaact agagaacaaa tgttaacaaa cagatttaat
     9061 gatcttttgg ataggatgga tataatgttt ggttctacag gctctgctga catagaagaa
     9121 tggatggctg gagttgcttg gctacactgt ttgttgccca aaatggattc agtggtgtat
     9181 gactttttaa aatgcatggt gtacaacatt cctaaaaaaa gatactggct gtttaaagga
     9241 ccaattgata gtggtaaaac tacattagca gctgctttgc ttgaattatg tggggggaaa
     9301 gctttaaatg ttaatttgcc cttggacagg ctgaactttg agctaggagt agctattgac
     9361 cagtttttag tagtttttga ggatgtaaag ggcactggag gggagtccag agatttgcct
     9421 tcaggtcagg gaattaataa cctggacaat ttaagggatt atttggatgg cagtgttaag
     9481 gtaaacttag aaaagaaaca cctaaataaa agaactcaaa tatttccccc tggaatagtc
     9541 accatgaatg agtacagtgt gcctaaaaca ctgcaggcca gatttgtaaa acaaatagat
     9601 tttaggccca aagattattt aaagcattgc ctggaacgca gtgagttttt gttagaaaag
     9661 agaataattc aaagtggcat tgctttgctt cttatgttaa tttggtacag acctgtggct
     9721 gagtttgctc aaagtattca gagcagaatt gtggagtgga aagagagatt ggacaaagag
     9781 tttagtttgt cagtgtatca aaaaatgaag tttaatgtgg ctatgggaat tggagtttta
     9841 gattggctaa gaaacagtga tgatgatgat gaagacagcc aggaaaatgc tgataaaaat
     9901 gaagatggtg gggagaagaa catggaagac tcagggcatg aaacgggcat tgattcacag
     9961 tcccaaggct catttcaggc ccctcagtcc tcacagtctg ttcatgatca taatcagcca
    10021 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    10081 gaaacataaa tcgatgctag tcgagctcaa gcttcgaatt ccgccccccc cccctaacgt
    10141 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
    10201 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
    10261 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
    10321 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
    10381 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
    10441 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
    10501 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
    10561 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
    10621 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
    10681 tgataatacc atggctagac tggacaagag caaagtcata aacggcgctc tggaattact
    10741 caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
    10801 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
    10861 cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga
    10921 ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc
    10981 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
    11041 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
    11101 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
    11161 aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt
    11221 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgcggatt
    11281 agaaaaacaa cttaaatgtg aaagtgggtc gccaaaaaag aagagaaagg tcgacggcgg
    11341 tggtgctttg tctcctcagc actctgctgt cactcaagga agtatcatca agaacaagga
    11401 gggcatggat gctaagtcac taactgcctg gtcccggaca ctggtgacct tcaaggatgt
    11461 atttgtggac ttcaccaggg aggagtggaa gctgctggac actgctcagc agatcgtgta
    11521 cagaaatgtg atgctggaga actataagaa cctggtttcc ttgggttatc agcttactaa
    11581 gccagatgtg atcctccggt tggagaaggg agaagagccc tggctggtgg agagagaaat
    11641 tcaccaagag acccatcctg attcagagac tgcatttgaa atcaaatcat cagtttaagg
    11701 atctcgacta gctagtagct agctagctag tcgagctcaa gcttcgaatt cccgggacta
    11761 gtcatatgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    11821 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    11881 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
    11941 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
    12001 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
    12061 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
    12121 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
    12181 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
    12241 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
    12301 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
    12361 tcggtacgta tggccaggta cctttaagac caatgactta caaggcagct gtagatctta
    12421 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
    12481 atctcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
    12541 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
    12601 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
    12661 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
    12721 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
    12781 gatcgatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
    12841 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    12901 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    12961 ttagtcagtg tggaaaatct ctagcagcat ctagcatgca tcagccatac cacatttgta
    13021 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    13081 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    13141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    13201 aaactcatca atgtatctta acgcgttaac tttaaataat gccaattatt taaagttaat
    13261 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
    13321 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    13381 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    13441 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taggccggcc
    13501 atatttgtca ccggactgcg gccgcgactc tagatcataa tcagcccggg ggatcagcct
    13561 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    13621 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    13681 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    13741 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggaa ccagctgggg
    13801 cgcgataact tcgtatagga gactttatac gaagttatgc gccattctag ttgtggtttg
    13861 tccaaactca tcaatgtatc ttatcatgtc tggatcccat cacaaagctc tgacctcaat
    13921 cctatagaaa ggaggaatga gccaaaattc acccaactta ttgtgggaag cttgtggaag
    13981 gctactcgaa atgtttgacc caagttaaac aatttaaagg caatgctacc aaatactaat
    14041 tgagtgtatg ttaacttctg acccactggg aatgtgatga aagaaataaa agctgaaatg
    14101 aatcattctc tctactatta ttctgatatt tcacattctt aaaataaagt ggtgatccta
    14161 actgacctta agacagggaa tctttactcg gattaaatgt caggaattgt gaaaaagtga
    14221 gtttaaatgt atttggctaa ggtgtatgta aacttccgac ttcaactgta ggggatcctc
    14281 tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    14341 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    14401 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    14461 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    14521 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    14581 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    14641 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    14701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    14761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    14821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    14881 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
    14941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    15001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    15061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    15121 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    15181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    15241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    15301 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    15361 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    15421 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    15481 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    15541 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    15601 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    15661 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    15721 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    15781 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    15841 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    15901 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    15961 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    16021 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    16081 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    16141 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    16201 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    16261 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    16321 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    16381 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    16441 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    16501 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
