LOCUS       sequence-121192-         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with C-term eGFP
            8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G04
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(156..179)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(231..252)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tacccaacac acggacacat gcacgaacac gcatgcagag agatcataca tcatctatag
       61 agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta caaccgagaa ggcgacgtga
      121 aaggaacaag aaaggaggag gagggcggcc gcttagtggt gatggtggtg atggtggtga
      181 ccagaggtct tgtatagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      241 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
      301 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      361 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
      421 atgccgttct tctgcttgtc agccatgata tagacgttgt ggctgttgta gttgtactcc
      481 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      541 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tct
//
