LOCUS       sequence-121193-        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  attR4-attR3 Destination cassette in Tol1 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pToneDest
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(427..550)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(783..800)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(783..799)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(792..814)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1008..1027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
       61 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgata
      121 cagtagaaat tacagaaact ttatcacgtt tagtaagtat agaggctgaa aatccagatg
      181 aagccgaacg acttgtaaga gaaaagtata agagttgtga aattgttctt gatgcagatg
      241 attttcagga ctatgacact agcgtatatg aataggtaga tgtttttatt ttgtcacaca
      301 aaaaagaggc tcgcacctct ttttcttatt tctttttatg atttaatacg gcattgagga
      361 caatagcgag taggctggat acgacgattc cgtttgagaa gaacatttgg aaggctgtcg
      421 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      481 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc aacttttcta
      541 tacaaagttg atagccctgc agggatatcg ctagcgggcc cctcgagggt tccatcccaa
      601 tggcgcgcgg ccaggttcag gctgttacaa acttacaaac tgtgacataa gtaaaccaat
      661 agtggtgcat attaatatat tacaaagggc tgagccgccg gtgccgcccc ccttcagttt
      721 tccgccccag gccaccgccc ggacggcccg tgcctagaac cgctactggc taaagggaat
      781 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      841 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      901 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
      961 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     1021 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     1081 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
//
