LOCUS       sequence-121195-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  attR4-attR3 Destination cassette in Tol1 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pToneDest
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(47..149)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(295..418)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    440..574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(464..483)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     518..537
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 catgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
       61 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
      121 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
      181 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
      241 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttacgg ccgccatagt
      301 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
      361 aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata catagttgat
      421 aaggcgcgcc aggcctgtag atccagacat gataagatac attgatgagt ttggacaaac
      481 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      541 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      601 gtttcaggtt cagggggagg tgtgggaggt tttttaatta aaacatttca gttgacgaag
      661 acaaacaaag ttctgttgtg actatggggg ggggggg
//
