LOCUS       sequence-121197-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cloning of sgRNAs for expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLsgRNA-OsU3m/LacZ
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(2..24)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     124..143
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     337..359
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     351..368
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     352..368
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(402..784)
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
ORIGIN
        1 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
       61 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
      121 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
      181 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
      241 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      301 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      361 cggccagtgc caagcttagt atgttatctt cagaggtctc ttgccacgga tcatctgcac
      421 aactctttta aaccagcttt gatctatgtg gatagccgag gtggtactaa tactagcctt
      481 tgttgtcgtc caattgcgta atgggccggc ccatactgca atacatgtcc tgaaaggctt
      541 catggcccac tacgaaatgc ttttctccta cagtttatct tactccttca catcacacgt
      601 ggtttccaac gtacccagtg ttcccggctt ccagcatttg ctggtagcac cagtagaaga
      661 cgcctgtctt gtgctatggt ccctgactgc acatctgatt cctccaagat ccatgcatgc
      721 ctgataactt taagttgctt cagaagaact ttaagtgatc tgttcgtatg tttaaagatt
      781 cctttagcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      841 attacgccag ctggcgaa
//
