LOCUS       sequence-121209-        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini Tol2 backbone with att4-attR2 cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  TITLE     Other Lawson Lab plasmids
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pminTolR4-R2
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(122..245)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    262..396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(286..305)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     340..359
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(568..755,756..767)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(779..798)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(780..798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(805..822)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(805..821)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(814..836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1049..1068)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
       61 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
      121 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
      181 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      241 agttgatgta ccatcgatga tgatccagac atgataagat acattgatga gtttggacaa
      301 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      361 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
      421 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
      481 tgtggtatgg ctgattatga tcctctagat cgtgcatgct tccgcggtta gatctattac
      541 cctgttatcc ctagggaaaa tagaatgaag tgatctccaa aaaataagta ctttttgact
      601 gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa
      661 aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac
      721 ttaagtacag taatcaagta aaattactca agtactttac acctctgggc ccaattcgcc
      781 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
      841 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      901 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      961 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1021 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     1081 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccc
//
