LOCUS       sequence-121210-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini Tol2 backbone with att4-attR2 cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  TITLE     Other Lawson Lab plasmids
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pminTolR4-R2
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(103..109,110..127,128..133))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(158..281)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    join(314..451,452..463)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(514..532)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(515..532)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(550..566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(550..566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(563..585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    574..590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(598..604,605..622,623..628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    643..664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(781..798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
      121 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
      181 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      241 atcattttac gtttctcgtt caacttttct atacaaagtt gattcgagat taccctgtta
      301 tccctacttc cttaatactc aagtacaatt ttaatggagt acttttttac ttttactcaa
      361 gtaagattct agccagatac ttttactttt aattgagtaa aattttccct aagtacttgt
      421 actttcactt gagtaaaatt tttgagtact ttttacacct ctgctcgacc atatgggaga
      481 gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc
      541 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      601 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      661 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      721 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      781 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      841 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      901 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      961 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtc
//
