LOCUS       sequence-121213-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry clone with activated Dusp6 (S159/167A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND
  TITLE     Vegfa signals through ERK to promote angiogenesis, but not artery 
            differentiation.
  JOURNAL   Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30.
  PUBMED    27578780
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3796-3805. Epub 2016 Aug 30"
COMMENT     Sequence Label: p3E-Dusp6DM_S159/197A
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..31
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..163
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctatgaccat gtaatacgac tcactatagg ggatatcgcg gccgcgttaa cgctaccatg
       61 gagctccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      121 aagcaatgct tttttataat gccaactttg tataataaag ttgttacgtg gactcgagag
      181 ggtccaactg aaaaacgttg tgattggttg gtgtggtgaa gtacagtggc tgggttgggg
      241 ccggcacacg gttatcacac ggactcttga gtcctaacgt gcgctcaaag tccaagagtt
      301 gacccatgaa gttaaagttg ggcgagatgt tcgacttctt cattttgaca atgtcataag
      361 catcgttcat ggacaggttg agcttctgca tgaggtacgc cactgttaca gtgacagaac
      421 gactgatgcc tgcaaggcag tgaacaagca cgccacactt cagtccacgg gcctcatcaa
      481 taaagctgat ggcttcaggg aaaaactgtg agaggttctg gctccagtga tcagagatgg
      541 gaatctgctt gtacttaaac tccccggcat tctcgaacat gttggggaga ttaggggtca
      601 cgttcaagat gtacttgatg ccaaactcct ccaggatatc caggtttgtg gagtccttag
      661 cacagcccag atacagatgt ggaaggatct ccacggggaa tgagggctgg gggttggaaa
      721 ggggtgcgcc atctgagtca gttgcactgc ttgggtctcg gtcgatatcc gactcgatgt
      781 ccgaggagtc agagctgatt ctgagccctc cgagacccag gacctgggag gttggtgcac
      841 tgctgcttga cgaaccgtcg aggttcgtct cacacattgc gggaaaatca gtttgaaatt
      901 tgctgaagcc accctcgaga tagaaagcct tgtagccctc gtccttcatt ctcctcagca
      961 gtaaacccaa cacggagccg ccgtcgatgt tttcattcca ctcgcg
//
