LOCUS       sequence-121214-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with C-term 
            8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G02
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(167..190)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    192..316
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        463..565
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(632..651)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttagtgg tgatggtggt
      181 gatggtggtg caccactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata
      241 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
      301 atatccagtc actatgaagc ttgctaagtt ggcagcatca cccgacgcac tttgcgccga
      361 ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga
      421 taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgcaagagg
      481 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
      541 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      601 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      661 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      721 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      781 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
      841 caccgttttc catgagcaaa ctgaaaccgt tttcatcgct ctggagtgaa t
//
