LOCUS       sequence-121215-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol1 expression plasmid with UAS-driven myc tagged nlsTdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pTol1-UAS-nlstdTomato
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..57)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(91..120)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(130..159)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(169..198)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(208..237)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(247..276)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      303..312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(315..339)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        339..357
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     394..410
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     394..410
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(760..776)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(761..777)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(801..820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(802..820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(822..842)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1075..1092)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1075..1091)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcatggaa ttcgagatct ctaaattcaa gtcctcttca gaaatgagct tttgctccat
       61 ggtgaggtcg cccaagctct ccatttcatt caagtcctct tcagaaatga gcttttgctc
      121 catttcattc aagtcctctt cagaaatgag cttttgctcc atttcattca agtcctcttc
      181 agaaatgagc ttttgctcca tttcattcaa gtcctcttca gaaatgagct tttgctccat
      241 ttcattcaag tcctcttcag aaatgagctt ttgctccata gctttaaatc gatgggatcc
      301 agccatggtg gcggagcctg cttttttgta caaacttgaa ttaaccctca ctaaagggaa
      361 caaaagctgg agctccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg
      421 caggaattcg tgtggaggag ctcaaagtga ggctgagacg cgatgggatc cattatatac
      481 cctctagagt cgacccttgc cggaggacag tactccgcca gcccggagga cagtactccg
      541 ccttgcgtcg gaggacagta ctccgacctc tagtgtcgga ggacagtact ccgaccttgc
      601 cggaggacag tactccgcag cccggaggac agtactccgc cttgcgtcgg aggacagtac
      661 tccgacctct agtgtcggag gacagtactc cgaccttgcc ggaggacagt actccgccag
      721 cccggaggac agtactccgc cttggaggcc taagcttatc gataccgtcg acctcgaggg
      781 ggggcccggt acccaattcg ccctatagtg agtcgtatta ccaacttttc tatacaaagt
      841 tgatagccct gcagggatat cgctagcggg cccctcgagg gttccatccc aatggcgcgc
      901 ggccaggttc aggctgttac aaacttacaa actgtgacat aagtaaacca atagtggtgc
      961 atattaatat attacaaagg gctgagccgc cggtgccgcc ccccttcagt tttccgcccc
     1021 aggccaccgc ccggacggcc cgtgcctaga accgctactg gctaaaggga attcactggc
     1081 cgtcgtttta ca
//
