LOCUS       sequence-121216-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol1 expression plasmid with UAS-driven myc tagged nlsTdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pTol1-UAS-nlstdTomato
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..40
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(93..149)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(245..268)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(443..462)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     712..730
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 caggcctggc gcgccttatc aactatgtat aataaagttg ctcgagacta gttacgtagg
       61 cgcgccccac tttgtacaag aaagctgggt ggaggaccgg ggttttcttc cacgtctcct
      121 gcttgcttta acagagagaa gttcgtggct ccggatcctc ccttgtacag ctcgtccatg
      181 ccgtacagga acaggtggtg gcggccctcg gagcgctcgt actgttccac gatggtgtag
      241 tcctcgttgt gggaggtgat gtccagcttg gtgtccacgt agtagtagcc gggcagttgc
      301 acgggcttct tggccatgta gatggtcttg aactccacca ggtagtggcc gccgtccttc
      361 agcttcaggg cctggtggat ctcgcccttc agcacgccgt cgcgggggta caggcgctcg
      421 gtggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcggg ggggaagttg
      481 gtgccgcgca tcttcacctt gtagatcagc gtgccgtcct gcagggagga gtcctgggtc
      541 acggtcacca gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
      601 aaggacagct tcttgtaatc ggggatgtcg gcggggtgct tcacgtacgc cttggagccg
      661 tacatgaact ggggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc
      721 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
      781 aactcgtggc cgttcatgga gccctccatg cgcaccttga agcgcatgaa ctctttgatg
      841 acggccatgt tgttgtcctc ggaggaggcg gtgccggagc tgccgctgcc ggtgctgccg
      901 gtgccatgcc ccaggaacag gtggtggcgg ccctcggagc gctcgtactg ttcacaatgt
      961 gta
//
