LOCUS       sequence-121219-        7446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep 
            expression construct. Note that an improved version is available: 
            Addgene plasmid# 92307. See comments for details.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Zinn E, Pacouret S, Khaychuk V, Turunen HT, Carvalho LS, 
            Andres-Mateos E, Shah S, Shelke R, Maurer AC, Plovie E, Xiao R, 
            Vandenberghe LH
  TITLE     In Silico Reconstruction of the Viral Evolutionary Lineage Yields a 
            Potent Gene Therapy Vector.
  JOURNAL   Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 
            10.1016/j.celrep.2015.07.019.
  PUBMED    26235624
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 
            10.1016/j.celrep.2015.07.019."
COMMENT     Sequence Label: pAnc80L65
FEATURES             Location/Qualifiers
     source          1..7446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2373..2384
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(2862..2878)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2862..2878)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3023..3040)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3194..3782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3274..3293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4013..4040)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4059..4874)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4782..4801
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4866..4885
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(5091..5118)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(5154..5609)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5291..5312)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5503..5522
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5736..5758
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5750..5767
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5751..5767
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5801..5817
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5802..5818
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttcgatc aactacgcag acaggtacca aaacaaatgt tctcgtcacg tgggcatgaa
       61 tctgatgctg tttccctgca gacaatgcga gagaatgaat cagaattcaa atatctgctt
      121 cactcacgga cagaaagact gtttagagtg ctttcccgtg tcagaatctc aacccgtttc
      181 tgtcgtcaaa aaggcgtatc agaaactgtg ctacattcat catatcatgg gaaaggtgcc
      241 agacgcttgc actgcctgcg atctggtcaa tgtggatttg gatgactgca tctttgaaca
      301 ataaatgatt taaatcaggt atggctgccg atggttatct tccagattgg ctcgaggaca
      361 acctctctga gggcattcgc gagtggtggg acttgaaacc tggagccccg aaacccaaag
      421 ccaaccagca aaagcaggac gacggccggg gtctggtgct tcctggctac aagtacctcg
      481 gacccttcaa cggactcgac aagggggagc ccgtcaacgc ggcggacgca gcggccctcg
      541 agcacgacaa ggcctacgac cagcagctca aagcgggtga caatccgtac ctgcggtata
      601 accacgccga cgccgagttt caggagcgtc tgcaagaaga tacgtctttt gggggcaacc
      661 tcgggcgagc agtcttccag gccaagaagc gggttctcga acctctcggt ctggttgagg
      721 aaggcgctaa gacggctcct ggaaagaaga ggccggtaga gcaatcaccc caggaaccag
      781 actcctcttc gggcatcggc aagaaaggcc agcagcccgc gagaaagaga ctcaactttg
      841 ggcagacagg cgactcagag tcagtgcccg accctcaacc actcggagaa ccccccgcag
      901 ccccctctgg tgtgggatct aatacaatgg ctgcaggcgg tggcgctcca atggcagaca
      961 ataacgaagg cgccgacgga gtgggtaacg cctcaggaaa ttggcattgc gattccacat
     1021 ggctgggcga cagagtcatc accaccagca cccgaacctg ggccctcccc acctacaaca
     1081 accacctcta caagcaaatc tccagccaat cgggaggcag caccaacgac aacacctact
     1141 tcggctacag caccccctgg gggtattttg actttaacag attccactgc cacttctcac
     1201 cacgtgactg gcagcgactc atcaacaaca actggggatt ccggcccaag aagctcaact
     1261 tcaagctctt caacatccag gtcaaggagg tcacgacgaa tgatggcacc acgaccatcg
     1321 ccaataacct taccagcacg gttcaggtct ttacggactc ggaataccag ctcccgtacg
     1381 tcctcggctc tgcgcaccag ggctgcctgc ctccgttccc ggcggacgtc ttcatgattc
     1441 ctcagtacgg gtacctgact ctgaacaatg gcagtcaggc cgtgggccgt tcctccttct
     1501 actgcctgga gtactttcct tctcaaatgc tgagaacggg caacaacttt cagttcagct
     1561 acacgtttga ggacgtgcct tttcacagca gctacgcgca cagccaaagc ctggaccggc
     1621 tgatgaaccc cctcatcgac cagtacctgt actacctgtc tcggactcag accacgagtg
     1681 gtaccgcagg aaatcggacg ttgcaatttt ctcaggccgg gcctagtagc atggcgaatc
     1741 aggccaaaaa ctggctaccc gggccctgct accggcagca acgcgtctcc aagacaacca
     1801 atcaaaataa caacagcaac tttgcctgga ccggtgccac caagtatcat ctgaatggca
     1861 gagactctct ggtaaatccc ggtcccgcta tggcaaccca caaggacgac gaagacaaat
     1921 tttttccgat gagcggagtc ttaatatttg ggaaacaggg agctggaaat agcaacgtgg
     1981 accttgacaa cgttatgata accaacgagg aagaaattaa aaccaccaac ccagtggcca
     2041 cagaagagta cggcacggtg gccactaacc tgcaatcggc caacaccgct cctgctacag
     2101 ggaccgtcaa cagtcaagga gccttacctg gcatggtctg gcaggaccgg gacgtgtacc
     2161 tgcagggtcc tatctgggcc aagattcctc acacggacgg acactttcat ccctcgccgc
     2221 tgatgggagg ctttggactg aaacacccgc ctcctcagat cctgattaag aatacacctg
     2281 ttcccgcgaa tcctccaact accttcagtc cagctaagtt tgcgtcgttc atcacgcagt
     2341 acagcaccgg acaggtcagc gtggaaattg aatgggagct gcagaaagaa aacagcaaac
     2401 gctggaaccc agagattcaa tacacttcca actacaacaa atctacaaat gtggactttg
     2461 ctgttgacac aaatggcgtt tattctgagc ctcgccccat cggcacccgt tacctcaccc
     2521 gtaatctgta aactagtttg cttgttaatc aataaaccgt ttaattcgtt tcagttgaac
     2581 tttggtctct gcgtatttct ttcttatcta gtttccatgc tctagaggtc ctgtattaga
     2641 ggtcacgtga gtgttttgcg acattttgcg acaccatgtg gtcacgctgg gtatttaagc
     2701 ccgagtgagc acgcagggtc tccattttga agcgggaggt ttgaacgcgc agccgccaag
     2761 ccgaattctg cagatatcca tcacactggc ggccgctcga ctagagcggc cgccaccgcg
     2821 gtggagctcc agcttttgtt cgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     2881 tgaaattcca cagcctgggg tgcctaattg cgttgcgctc actgcccgct ttccagtcgg
     2941 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3001 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3061 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agaaataata
     3961 aaaaagccgg attaataatc tggcttttta tattctctct ctagtatata aacgcagaaa
     4021 ggcccacccg aaggtgagcc agtgtgactc tagtattatt agaaaaactc atcgagcatc
     4081 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     4141 ttctgtaatg aaggagaaaa ctcaccgagg cagttccaaa gaatggcaag gtcctggtaa
     4201 cggtctgcga ttccgacccg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     4261 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaag
     4321 agcttgtgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     4381 tcactcgcat caaccaaacc gttattcatg cgtgattgcg cctgagcaag acgaaataca
     4441 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gtaaccggcg caggaacacg
     4501 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaaggct
     4561 gttttcccag gaatcgcggt ggtgagtaac cacgcatcat caggagtacg gataaaatgc
     4621 ttgatggtcg ggagaggcat aaactccgtc agccagttga gacggaccat ctcatctgta
     4681 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     4741 ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     4801 ccatataaat cagcgtccat gttggagttt aagcgcggac gggagcaaga cgtttcccgt
     4861 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     4921 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     4981 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agctctagta aaataataaa
     5041 aaagccggat taataatctg gctttttata ttctctctct agtatataaa cgcagaaagg
     5101 cccacccgaa ggtgagccag tgtgacggca catttccccg aaaagtgcca cctaaattgt
     5161 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     5221 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     5281 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     5341 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     5401 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     5461 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     5521 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     5581 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     5641 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     5701 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     5761 ggccagtgag cgcgcggcga attgggtacc gggccccccc tcgaggtcga cggtatcgta
     5821 tttaagcccg agtgagcacg cagggtctcc attttgaagc gggaggtttg aacgcgcagc
     5881 cgccatgccg gggttttacg agattgtgat taaggtcccc agcgaccttg acgagcatct
     5941 gcccggcatt tctgacagct ttgtgaactg ggtggccgag aaggaatggg agttgccgcc
     6001 agattctgac atggatctga atctgattga gcaggcaccc ctgaccgtgg ccgagaagct
     6061 gcagcgcgac tttctgacgg aatggcgccg tgtgagtaag gccccggagg ctcttttctt
     6121 tgtgcaattt gagaagggag agagctactt ccacatgcac gtgctcgtgg aaaccaccgg
     6181 ggtgaaatcc atggttttgg gacgtttcct gagtcagatt cgcgaaaaac tgattcagag
     6241 aatttaccgc gggatcgagc cgactttgcc aaactggttc gcggtcacaa agaccagaaa
     6301 tggcgccgga ggcgggaaca aggtggtgga tgagtgctac atccccaatt acttgctccc
     6361 caaaacccag cctgagctcc agtgggcgtg gactaatatg gaacagtatt taagcgcctg
     6421 tttgaatctc acggagcgta aacggttggt ggcgcagcat ctgacgcacg tgtcgcagac
     6481 gcaggagcag aacaaagaga atcagaatcc caattctgat gcgccggtga tcagatcaaa
     6541 aacttcagcc aggtacatgg agctggtcgg gtggctcgtg gacaagggga ttacctcgga
     6601 gaagcagtgg atccaggagg accaggcctc atacatctcc ttcaatgcgg cctccaactc
     6661 gcggtcccaa atcaaggctg ccttggacaa tgcgggaaag attatgagcc tgactaaaac
     6721 cgcccccgac tacctggtgg gccagcagcc cgtggaggac atttccagca atcggattta
     6781 taaaattttg gaactaaacg ggtacgatcc ccaatatgcg gcttccgtct ttctgggatg
     6841 ggccacgaaa aagttcggca agaggaacac catctggctg tttgggcctg caactaccgg
     6901 gaagaccaac atcgcggagg ccatagccca cactgtgccc ttctacgggt gcgtaaactg
     6961 gaccaatgag aactttccct tcaacgactg tgtcgacaag atggtgatct ggtgggagga
     7021 ggggaagatg accgccaagg tcgtggagtc ggccaaagcc attctcggag gaagcaaggt
     7081 gcgcgtggac cagaaatgca agtcctcggc ccagatagac ccgactcccg tgatcgtcac
     7141 ctccaacacc aacatgtgcg ccgtgattga cgggaactca acgaccttcg aacaccagca
     7201 gccgttgcaa gaccggatgt tcaaatttga actcacccgc cgtctggatc atgactttgg
     7261 gaaggtcacc aagcaggaag tcaaagactt tttccggtgg gcaaaggatc acgtggttga
     7321 ggtggagcat gaattctacg tcaaaaaggg tggagccaag aaaagacccg cccccagtga
     7381 cgcagatata agtgagccca aacgggtgcg cgagtcagtt gcgcagccat cgacgtcaga
     7441 cgcgga
//
