LOCUS       sequence-121220-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre under the control of the elongation factor-1 alpha 
            (EF1alpha) promoter; includes upstream Kozak modification to 
            increase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Le Y, Miller JL, Sauer B
  TITLE     GFPcre fusion vectors with enhanced expression.
  JOURNAL   Anal Biochem. 1999 Jun 1. 270(2):334-6.
  PUBMED    10334853
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 1999 Jun 1. 270(2):334-6."
COMMENT     Sequence Label: pBS513 EF1alpha-cre
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catttcaggt gtcgtgagga attggatcca gattcgaggg gcagagccga tcctgtacac
       61 tttacttaaa accattatct gagtgtgaaa tgtccaattt actgaccgta caccaaaatt
      121 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
      181 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt
      241 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg
      301 ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac
      361 atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca
      421 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg
      481 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa
      541 atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc
      601 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg
      661 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
      721 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
      781 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
      841 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat
      901 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg
      961 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
     1021 aagctggtgg ctggaccaat gtaaatattg t
//
