LOCUS       sequence-121221-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre under the control of the elongation factor-1 alpha 
            (EF1alpha) promoter; includes upstream Kozak modification to 
            increase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Le Y, Miller JL, Sauer B
  TITLE     GFPcre fusion vectors with enhanced expression.
  JOURNAL   Anal Biochem. 1999 Jun 1. 270(2):334-6.
  PUBMED    10334853
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 1999 Jun 1. 270(2):334-6."
COMMENT     Sequence Label: pBS513 EF1alpha-cre
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga
       61 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac
      121 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat
      181 tagccattaa cgcgtaaatg attgctataa ttatttgata tttatggtga catatgagaa
      241 aggatttcaa catcgacgga aaatatgtag tgctgtctgt aagcactaat attcagtcgc
      301 cagccgtcat tgtcactgta aagctgagcg atagaatgcc tgatattgac tcaatatccg
      361 ttgcgtttcc tgtcaaaagt atgcgtagtg ctgaacattt cgtgatgaat gccaccgagg
      421 aagaagcacg gcgcggtttt gctaaagtga tgtctgagtt tggcgaactc ttgggtaagg
      481 ttggaattgt cgagggatct cggaatggac cccaactgct cctgctccac cggtaagact
      541 cccgatcctt ggtctttaga ataccaagtt gggaccgcag agcggaatcc ccgagttgta
      601 gaggcttggc gggaataggc acctttagtt ggcgattcat tccggttctt tctagaatcc
      661 gctcttgcaa aagccttcat tagttacgag tattgtcgaa cgggtccttt ggcggggttg
      721 gggctaggat ttagacgcgc aaatgtccgg ttcctgatca cccagttagt ggggacatct
      781 gggttgagtc ccaggcatta ctaaacttac tgtgaattgc ttgaattaag aaagaggtga
      841 aggaccttta tgtcttggga ctcaaagaca taatccctga cttaacctgt gaggagaaaa
      901 gtggggctag gctccctgca gctccgagga ggacttagtg aactgagccg ggactcgtgg
      961 tgttggccac tgctgtaatg ctgcctccct catgctgtct tctttctcct ccccaggcgg
     1021 ctcctgcact tgcaccagct cctgcgcc
//
