LOCUS       sequence-121232-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cloning of sgRNAs for expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLsgRNA-OsU3m
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    78..99
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(114..119,120..137,138..144)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    152..168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     157..179
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     176..192
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     176..192
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        242..317
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        384..766
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     primer_bind     complement(800..817)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(800..816)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
       61 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      121 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      181 aacagctatg accatgatta cgaattcgag ctcggtaccc ggggatccag cgtgggtctc
      241 ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      301 tggcaccgag tcggtgcttt ttttcaagag cttggagtgg atggaatttt cctccgtttt
      361 acctgtggaa tcggcagcaa aggaaggaat ctttaaacat acgaacagat cacttaaagt
      421 tcttctgaag caacttaaag ttatcaggca tgcatggatc ttggaggaat cagatgtgca
      481 gtcagggacc atagcacaag acaggcgtct tctactggtg ctaccagcaa atgctggaag
      541 ccgggaacac tgggtacgtt ggaaaccacg tgtgatgtga aggagtaaga taaactgtag
      601 gagaaaagca tttcgtagtg ggccatgaag cctttcagga catgtattgc agtatgggcc
      661 ggcccattac gcaattggac gacaacaaag gctagtatta gtaccacctc ggctatccac
      721 atagatcaaa gctggtttaa aagagttgtg cagatgatcc gtggcaagag acctctgaag
      781 ataacatact aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
//
