LOCUS       sequence-121233-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD L448S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS509 (TJF032 L448S)
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    169..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(193..212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(194..212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(301..318)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc
      121 tgccatatgt atatctcctt cttatactta actaatatac taagatgggg aattgttatc
      181 cgctcacaat tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac
      241 gattactttc tgttcgactt aagcattatg cggccgcaag cttggatcct cattcattca
      301 gtgatgatga tggtgatgag aagaacctca ggctttattg tccactttgc cgcgcgcttc
      361 gtcacgtaat tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa
      421 ctccaccagc ttcggtactt tgtagcccgt gagctgacgg cggcaaaagg tcaccagtga
      481 ctcttcggta agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga
      541 gccggaaggt acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc
      601 ttcaatctcg ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc
      661 gacaatgcga gagaatcctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc
      721 atttttgatg atctcatctg tagcatccgg acgctgccag taacccagca tcacctgcgg
      781 tcctttgaca caaagctcac ccggttgacc cggtggtact tcattatcat catcatccac
      841 cagtttggct tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc
      901 atatgggtta acgctgacca gcggcgcaca ctcggtaagg ccatagcctt ccagcagata
      961 ctgtcctgtc agtttcaccc aacgctctgc caccacttgc tgcactggca tccctccgcc
     1021 tgcggaaaga tgcaaactgg agaaatccag ctgctggaac tcttt
//
