LOCUS       sequence-121239-         328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, hygromycin resistance for plant 
            selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201H Cas9
FEATURES             Location/Qualifiers
     source          1..328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gactctctta aggtagccaa aatcgatatc tagggataac agggtaatcg atcactagaa
       61 ttcggcgcgc cgggcccaac atggtggagc acgacactct cgtctactcc aagaatatca
      121 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
      181 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
      241 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
      301 cctctgccga cagtggtccc aaagatgg
//
