LOCUS       sequence-121241-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, hygromycin resistance for plant 
            selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201H Cas9
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..102)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acttccgttg tgtgatcagt ttggcgttca gcagctgccg ccaataattt ttcattttct
       61 tgacaacttc ttctgagggg acgttatcac tcttccctct atttttatcg gatcttgtca
      121 acactttatt atcaatagaa tcatctttga gaaaagactg gggcacgata tgatccacgt
      181 cgtagtcgga gagccgattg atgtccagtt cctgatccac gtacatgtcc ctgccgttct
      241 gcaggtagta caggtagagc ttctcattct gaagctgggt gttttcaact gggtgttcct
      301 taaggatttg ggaccccagt tcttttatac cctcttcaat cctcttcatc ctttccctac
      361 tgttcttctg tcccttctgg gtagtttggt tctctcgggc catctcgata acgatattct
      421 cgggcttatg ccttcccatt actttgacga gttcatccac gaccttaacg gtctgcagta
      481 ttcccttttt gatagctggg ctacctgcaa gattagcgat gtgctcgtga agactgtccc
      541 cctggccaga aacttgtgct ttctggatgt cctccttaaa ggtgagagag tcatcatgga
      601 tcaactgcat gaagttccgg ttggcaaatc catcggactt aagaaaatcc aggattgtct
      661 ttccactctg cttgtctcgg atcccattga tcagttttct tgacagccgc ccccatcctg
      721 tatatcggcg cctcttgagc tgtttcatga cttt
//
