LOCUS       sequence-121242-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cloning of sgRNAs for expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     Sequence Label: pYLsgRNA-AtU3d/LacZ
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..92
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(107..112,113..130,131..137)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     169..185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     169..185
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        235..310
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(715..731)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
       61 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      181 atgaccatga ttacgaattc gagctcggta cccggggatc cagcgtgggt ctcggtttta
      241 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
      301 gagtcggtgc tttttttcaa gagcttggag tggatggaat tttcctccgt tttacctgtg
      361 gaatcggcag caaaggacgc gttgacattg taggactata ttgctctaat aaaggaggca
      421 gctatgctgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      481 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      541 gatcgccctt cccaacagtt gcgcagcctg aatggctaaa taagcttatg atttcttttt
      601 tcttacgaat tttgcgtccc acatcggtaa gcgagtgaag aaataactgc tttatatatg
      661 gctacaaagc accattggtc aagagacctc tgaagataac atactaagct tggcactggc
      721 cgtcgtttta caacgtcgtg actgg
//
