LOCUS       sequence-121247-        1153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD L448S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS509 (TJF032 L448S)
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..62)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttagcattat gcggccgcaa gcgttggatc ctcattcact atcagtgatg atgatggtga
       61 tgagaanaac ctcaggcttt attgtccact ttgccgcgcg cttcgtcacg taattctcgt
      121 cgcaaaattt ttccgacgtt agatttcggt aactcatcac gaaactccac cagcttcggt
      181 actttgtagc ccgtgagctg acggcggcaa aaggtcacca gtgactcttc ggtaagcgat
      241 ggatcttttt tcactacgaa gattttcacc gcttcaccac tggagccgga aggtacgcca
      301 acagccgcga cttcctgtac gccaggatgc tgcatgacga catcttcaat ctcgttggga
      361 tagacgttaa aaccggaaac cagaatcatg tcttttttac gatcgacaat gcgagagaat
      421 ccttcttcat ccatcaccgc gatgtcgccg gtgtgtaacc agccattttt gatgatctca
      481 tctgtagcat ccggacgctg ccagtaaccc agcatcacct gcggtccttt gacacaaagc
      541 tcacccggtt gacccggtgg tacttcatta tcatcatcat ccaccagttt ggcttccgtc
      601 gacggcaccg gcaaaccgat gctaccacta tgataatcaa tatcatatgg gttaacgctg
      661 accagcggcg cacactcggt aaggccatag ccttccagca gatactgtcc tgtcagtttc
      721 acccaacgct ctgccaccac ttgctgcact ggcatccctc cgcctgcgga aagatgcaga
      781 ctggagaaat ccagctgctg gaactcttta ttgttcagca acgcattgaa caaggtgtta
      841 acgcccgtga tagcggtaaa cggatatttc gctaactctt ttaccaaccc tggaatatcg
      901 cgcgggttag tgataagcag gttctgccca cccagttcga taaacagcag gcagttaatg
      961 gtcagggcaa aaatgtgata cagcggcagc gccgtcacca ccagctcttt gcccggatgc
     1021 aacagcggac cataggtcgc gttaacctgt tccaggttcg ccagcatatt gcggtgagtc
     1081 agcatcgcgc ctttcgccac accagtggtg ccgccggtgt attgcagaaa agctaaatct
     1141 tcccnggccc agt
//
