LOCUS       sequence-121248-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72114-BBa_J72104
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             131..139
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tttccgggcc tgtatccgcc ggatatggcc gagaaactgt actttacgag cgagccgtac
       61 gatcgtctgc aacaaatcca gcgtaaacag aatgtttggt acaagattgg tcgcgtgaat
      121 tgatgatctt taaggaggta aaaaaaatgg agaaccgtga gccaccactg ttgccagccc
      181 gttggagcag cgcctatgtc tcttattgga gcccgatgct gccggatgac cagctgacca
      241 gcggctattg ctggttcgac tatgaacgtg acatctgtcg tattgacggc ctgttcaatc
      301 cgtggagcga gcgtgatact ggttatcgcc tgtggatgtc ggaggttggt aatgcggcca
      361 gcggccgtac ctggaaacaa aaagtcgcct atggtcgtga gcgtaccgcc ctgggtgaac
      421 agctgtgtga gcgtccgctg gatgatgaga ctggcccttt tgccgaattg ttcctgccac
      481 gcgatgtcct gcgccgtctg ggtgcccgtc acattggccg tcgcgtggtt ctgggtcgcg
      541 aagcggacgg ttggcgttac cagcgcccag gtaaaggtcc gagcaccctg tacctggatg
      601 cggcgagcgg cactccactg cgcatggtca ccggcgatga agcgtcgcgt gcaagcctgc
      661 gtgattttcc gaatgtgagc gaggcggaga tcccggacgc ggttttcgcg gccaagcgct
      721 aaggatctcc aggcatca
//
