LOCUS       sequence-12125-e        7922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Teschemacher AG, Wang S, Raizada MK, Paton JF, Kasparov S
  TITLE     Area-specific differences in transmitter release in central 
            catecholaminergic neurons of spontaneously hypertensive rats.
  JOURNAL   Hypertension. 2008 Aug . 52(2):351-8.
  PUBMED    18606900
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Hypertension. 2008 Aug . 52(2):351-8."
COMMENT     Sequence Label: pXcX-PRS-EGFP
FEATURES             Location/Qualifiers
     source          1..7922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      260..269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(266..268,269..271,272..985)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(266..268,269..271,272..982)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(311..332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(572..591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     919..940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1025..1042)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1031..1255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_signal     1380..1530
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   1618..1720
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(1740..1758)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1826..1930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1931..1999,2000..2791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2149..2168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2962..3550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3451..3470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3704..3721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3736..3876
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3802..3821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3962..3984
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3978..4169)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agcttccgct agacaaatgt gattaccccc gctagacaaa tgtgattacc cgcgctagac
       61 aaatgtgatt accccgctag acaaatgtga ttaccccccg ctagacaaat gtgattaccc
      121 ccgctagaca aatgtgatta cccgcgctag acaaatgtga ttaccccgct agacaaatgt
      181 gattaccccc gaccagggca taaatggcca ggtgggacca gagagctcac cccagccgac
      241 tctagaggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      301 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      361 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      421 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      481 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      541 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      601 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      661 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      721 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      781 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      841 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
      901 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      961 cggcatggac gagctgtaca agtaaagcgg ccgctcgagc atgcatctag agctcgctga
     1021 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1081 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1141 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1201 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggaacca
     1261 gctagactga ctataataat aaaacgccaa ctttgacccg gaacgcggaa aacacctgag
     1321 aaaaacacct gggcgagtct ccacgtaaac ggtcaaagtc cccgcggccc tagacaaata
     1381 ttacgcgcta tgagtaacac aaaattattc agatttcact tcctcttatt cagttttccc
     1441 gcgaaaatgg ccaaatctta ctcggttacg cccaaattta ctacaacatc cgcctaaaac
     1501 cgcgcgaaaa ttgtcacttc ctgtgtacac cggcgcacac caaaaacgtc acttttgcca
     1561 catccgtcgc ttacatgtgt tccgccacac ttgcaacatc acacttccgc cacactacta
     1621 cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa actccacccc ctcattatca
     1681 tattggcttc aatccaaaat aaggtatatt attgatgatg aattcttgaa gacgaaaggg
     1741 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2161 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2461 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3721 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3781 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     3841 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     3901 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3961 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     4021 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     4081 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     4141 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     4201 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     4261 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     4321 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     4381 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     4441 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     4501 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     4561 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     4621 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
     4681 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     4741 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     4801 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     4861 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
     4921 cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga
     4981 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     5041 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     5101 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
     5161 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     5221 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     5281 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     5341 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     5401 ctgggttgaa ggctctcaag ggcatcggtc gaggacaggc ctctcaagtc tgtatacggg
     5461 gacacggaca gccttttcgt caccgagcgt ggacaccggc tcatggaaac cagaggtaag
     5521 aaacgcatca aaaagcatgg gggaaacctg gtttttgacc ccgaacggcc agagctcacc
     5581 tggctcgtgg aatgcgagac cgtctgcggg gcctgcggcg cggatgccta ctccccggaa
     5641 tcggtatttc tcgcgcccaa gctctacgcc ctcaaaagtc tgcactgccc ctcgtgcggc
     5701 gcctcctcca agggcaagct gcgcgccaag ggccacgccg cggaggggct ggactatgac
     5761 accatggtca aatgctacct ggccgacgcg cagggcgaag accggcagcg cttcagcacc
     5821 agcaggacca gcctcaagcg caccctggcc agcgcgcagc ccggagcgca ccccttcacc
     5881 gtgacccaga ctacgctgac gaggaccctg cgcccgtgga aagacatgac cctggcccgt
     5941 ctggacgagc accgactact gccgtacagc gaaagccgcc ccaacccgcg aaacgaggag
     6001 atatgctgga tcgagatgcc gtagagcagg tgaccgagct gtgggaccgc ctggaactgc
     6061 ttggtcaaac gctcaaaagc atgcctacgg cggacggtct caaaccgttg aaaaactttg
     6121 cttccttgca agaactgcta tcgctgggcg gcgagcgcct tctggcggat ttggtcaggg
     6181 aaaacatgcg agtcagggac atgcttaacg aagtggcccc cctgctcagg gatgacggca
     6241 gctgcagctc tcttaactac cagttgcacc cggtaatagg tgtgatttac gggcccaccg
     6301 gctgcggtaa gtcgcagctg ctcaggaacc tgctttcttc ccagctgatc tcccctaccc
     6361 cggaaaccgt tttcttcatc gccccgcagg tagacatgat ccccccatct gaactcaaag
     6421 cgtgggaaat gcaaatctgt gagggtaact acgcccctgg gccggatgga accattatac
     6481 cgcagtctgg caccctccgc ccgcgctttg taaaaatggc ctatgacgat ctcatcctgg
     6541 aacacaacta tgacgttagt gatcccagaa atatcttcgc ccaggccgcc gcccgtgggc
     6601 ccattgccat cattatggac gaatgcatgg aaaatcttgg aggtcacaag ggcgtctcca
     6661 agttcttcca cgcatttcct tctaagctac atgacaaatt tcccaagtgc accggataca
     6721 ctgtgctggt ggttctgcac aacatgaatc cccggaggga tatggctggg aacatagcca
     6781 acctaaaaat acagtccaag atgcatctca tatccccacg tatgcaccca tcccagctta
     6841 accgctttgt aaacacttac accaagggcc tgcccctggc aatcagcttg ctactgaaag
     6901 acatttttag gcaccacgcc cagcgctcct gctacgactg gatcatctac aacaccaccc
     6961 cgcagcatga agctctgcag tggtgctacc tccaccccag agacgggctt atgcccatgt
     7021 atctgaacat ccagagtcac ctttaccacg tcctggaaaa aatacacagg accctcaacg
     7081 accgagaccg ctggtcccgg gcctaccgcg cgcgcaaaac ccctaaataa agacagcaag
     7141 acacttgctt gatccaaatc caaacagagt ctggtttttt atttatgttt taaaccgcat
     7201 tgggagggga ggaagccttc agggcagaaa cctgctggcg cagatccaac agctgctgag
     7261 aaacgacatt aagttcccgg gtcaaagaat ccaattgtgc caaaagagcc gtcaacttgt
     7321 catcgcgggc ggatgaacgg gaagctgcac tgcttgcaag cgggctcagg aaagcaaagt
     7381 cagtcacaat cccgcgggcg gtggctgcag cggctgaagc ggcggcggag gctgcagtct
     7441 ccaacggcgt tccagacacg gtctcgtagg tcaaggtagt agagtttgcg ggcaggacgg
     7501 ggcgaccatc aatgctggag cccatcacat tctgacgcac cccggcccat gggggcatgc
     7561 gcgttgtcaa atatgagctc acaatgcttc catcaaacga gttggtgctc atggcggcgg
     7621 cggctgctgc aaaacagata caaaactaca taagaccccc accttatata ttctttccca
     7681 cccttaagcc acgcccacac atttcagtac ctcaatctgt atcttcatcg ctagagccaa
     7741 actcagcgcg ggtgcaggcc agcaccaagt gatcgggcct cagctcctcg gtcacatcca
     7801 gcatcacagg ctggttccta atatgtttac cgccacactc gcagggtctg cacctggtgc
     7861 gggtctcatc gtacctcagc accttccaga tcntctagcg atgtacgggc cagatatacg
     7921 cg
//
