LOCUS       sequence-121250-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of ER localized oxBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha 
            VV, Snapp EL
  TITLE     A palette of fluorescent proteins optimized for diverse cellular 
            environments.
  JOURNAL   Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670.
  PUBMED    26158227
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-9"; volume: "6:7670. doi"; pages: " 
            10.1038/ncomms8670"
COMMENT     Sequence Label: ERoxBFP
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           18..60
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             69..161
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVST"
     regulatory      171..180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(177..179,180..182,183..893)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(222..243)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accgtcagat ccgctagctt gttctttttg cagaagctca gaataaacgc tcaactttgg
       61 cagataccat ggacagcaaa ggttcgtcgc agaaagggtc ccgcctgctc ctgctgctgg
      121 tggtgtcaaa tctactcttg tgccagggtg tggtctccac cggaccggtc gccaccatgg
      181 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      241 acgtaaacgg ccacaagttc agcgtgaggg gcgagggcga gggcgatgcc accaacggca
      301 agctgaccct gaagttcatc agtactaccg gcaagctgcc cgtgccctgg cccaccctcg
      361 tgaccaccct gagccacggc gtgcaggtgt tcgcccgcta ccccgaccac atgaagcagc
      421 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      481 aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      541 accgcatcga gctgaagggc gtcgacttca aggaggacgg caacatcctg gggcacaagc
      601 tggagtacaa cttcaacagc cacaacatct atatcatggc cgtcaagcag aagaacggca
      661 tcaaggccaa cttcaagatc cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc
      721 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac agccactacc
      781 tgagcaccca gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      841 tggagttccg caccgccgcc gggatcactc tcggcatgga cgagctgtac aaggatgaa
//
